Aimsandobjectives: The frequent and unprescribed use of antibiotics has led to the development of resistance by microorganisms responsible for urinary tract infection (UTI). In order to facilitate the follow-up of this microbial resistance, the aim of this study was to determine the bacteria resistant phenotypes. Method: To achieve the following objectives, this study was conducted from June to August 2023. The isolation and identification were performed by standard methods why susceptibility testing was done by Kirby-Bauer disk diffusion technique according to CLSI guidelines. Double-disk synergy test was applied to determine the presence of extended-spectrum β-lactamase (ESBL) produced by bacteria. The Imipenem EDTA Combined Disc Test (CDT) for Metallo beta lactamase (MBL) screening, the D-zone test to detect macrolides, lincosamides and streptogramins type B (MLSB) and Meticillin resistant Staphylococcusaureus (MRS A) which was assessed using a Cefoxitin (30 μg) disc. Results: A total of 40 bacteria were identified with a prevalence of 37.03%. Overall, E.coli was the predominant isolate 14 (35%), followed by Staphylococcusaureus 10 (25%) and Klesbsiellapneumonia 4 (10%). Pseudomonasaeruginosa,Salmonellaarinosa and Enterobacter were the most sensible (100%) bacteria against ciprofloxin, ceftriaxone and cefixime. Almost all bacteria were resistant to Amoxicillin/clavulanic acid (>50%). The isolates were in the majority resistant to imipenem. ESBL-producing Enterobacteriaceae represented 25.92%, with a higher rate among E.coli. No MBL production was found among the isolates while 38.46% represented cMLSB, 15.38% represented iMLSB, 23.07% represented MSB and 23.07% represented MRSA. Conclusion: Clinical strains of UTI from Protestant Hospital of Ngaoundere exhibiting ESBL, cMLSB, iMLSB, MSB and MRSA. The regular updating of antibiotic resistance statistics of isolated strains allows for a better adaptation of probabilistic antibiotic therapy.
References
[1]
Stamm, W.E. and Norrby, S.R. (2001) Urinary Tract Infections: Disease Panorama and Challenges. The Journal of Infectious Diseases, 183, S1-S4. https://doi.org/10.1086/318850
[2]
Centers for Disease Control and Preventions (2021) Urinary Track Infection Basics. https://www.cdc.gov/uti/about/index.html
[3]
Gupta, K., Hooton, T.M. and Stamm, W.E. (2001) Increasing Antimicrobial Resistance and the Management of Uncomplicated Community-Acquired Urinary Tract Infections. Annals of Internal Medicine, 135, 41-50. https://doi.org/10.7326/0003-4819-135-1-200107030-00012
[4]
Zeng, Z., Zhan, J., Zhang, K., Chen, H. and Cheng, S. (2022) Global, Regional, and National Burden of Urinary Tract Infections from 1990 to 2019: An Analysis of the Global Burden of Disease Study 2019. World Journal of Urology, 40, 755-763. https://doi.org/10.1007/s00345-021-03913-0
[5]
Saderi, H., Emadi, B. and Owlia, P. (2011) Phenotypic and Genotypic Study of Macrolide, Lincosamide and Streptogramin B (MLSB) Resistance in Clinical Isolates of Staphylococcus aureus in Tehran, Iran. Medical Science Monitor, 17, BR48-BR53. https://doi.org/10.12659/msm.881386
[6]
Akoachere, J.T.K., Yvonne, S., Akum, N.H. and Seraphine, E.N. (2012) Etiologic Profile and Antimicrobial Susceptibility of Community-Acquired Urinary Tract Infection in Two Cameroonian Towns. BMC Research Notes, 5, Article No. 219. https://doi.org/10.1186/1756-0500-5-219
[7]
Mwang’onde, B.J. and Mchami, J.I. (2022) The Aetiology and Prevalence of Urinary Tract Infections in Sub-Saharan Africa: A Systematic Review. Journal of Health & Biological Sciences, 10, 1-7. https://doi.org/10.12662/2317-3076jhbs.v10i1.4501.p1-7.2022
[8]
Smelov, V., Naber, K. and Bjerklund Johansen, T.E. (2016) Improved Classification of Urinary Tract Infection: Future Considerations. European Urology Supplements, 15, 71-80. https://doi.org/10.1016/j.eursup.2016.04.002
[9]
Behzadi, P. (2018) Uropathogenic Escherichia coli and Fimbrial Adhesins Virulome. In: Jarzembowski, T., et al., Eds., Urinary Tract Infection—The Result of the Strength of the Pathogen, or the Weakness of the Host, InTech, 65-83. https://doi.org/10.5772/intechopen.71374
Petca, R.C., Popescu, R.I, Mareș, C., Petca, A., Mehedințu, C. Sandu, I. and Măru, N. (2019) Antibiotic Resistance Profile of Common Uropathogens Implicated in Urinary Tract Infections in Romania. Farmacia, 67, 994-1004. https://doi.org/10.31925/farmacia.2019.6.9
[12]
CA-SFM/EUCAST (2022) Comité de l’Antibiogramme de la Société Française de Microbiologie/European Committee on Antimicrobien Susceptibility Testing, Recommandations 2022 V.1.0 mai.
[13]
Clinical and Laboratory Standards Institute (CLSI) (2008).
[14]
Bauer, A.W., Kirby, W.M.M., Sherris, J.C. and Turck, M. (1966) Antibiotic Susceptibility Testing by a Standardized Single Disk Method. American Journal of Clinical Pathology, 45, 493-496. https://doi.org/10.1093/ajcp/45.4_ts.493
[15]
Singh, T., Poonia, S. and Tsering, D. (2014) Antibiotic Susceptibility Profile of Bacteria Isolated from Natural Sources of Water from Rural Areas of East Sikkim. Indian Journal of Community Medicine, 39, 156-160. https://doi.org/10.4103/0970-0218.137152
[16]
Dirar, M., Bilal, N., Ibrahim, M.E. and Hamid, M. (2020) Resistance Patterns and Phenotypic Detection of β-Lactamase Enzymes among Enterobacteriaceae Isolates from Referral Hospitals in Khartoum State, Sudan. Cureus, 12, e7260. https://doi.org/10.7759/cureus.7260
Saderi, H., Emadi, B. and Owlia, P. (2011) Phenotypic and Genotypic Study of Macrolide, Lincosamide and Streptogramin B (MLSB) Resistance in Clinical Isolates of Staphylococcus aureus in Tehran, Iran. Medical Science Monitor, 17, BR48-BR53. https://doi.org/10.12659/msm.881386
[19]
Jombo, G., Egah, D., Banwat, E. and Ayeni, J. (2007) Nosocomial and Community Acquired Urinary Tract Infections at a Teaching Hospital in North Central Nigeria: Findings from a Study of 12,458 Samples. Nigerian Journal of Medicine, 15, 230-236. https://doi.org/10.4314/njm.v15i3.37219
[20]
Vasudevan, R. (2014) Urinary Tract Infection: An Overview of the Infection and the Associated Risk Factors. Journal of Microbiology & Experimentation, 1, 42-54. https://doi.org/10.15406/jmen.2014.01.00008
[21]
Djim-Adjim-Ngana, K., Mbiakop, B.W., Oumar, L.A., Munshili Njifon, H.L., Tchinda Fossi, C., Enyegue, E.L.E., et al. (2023) Phenotypic Characterization and Epidemiology of Extended-Spectrum Β-Lactamase-Producing Enterobacteriaceae Strains from Urinary Tract Infections in Garoua, Cameroon. Frontiers in Public Health, 11, Article ID: 1187934. https://doi.org/10.3389/fpubh.2023.1187934
[22]
Rasamiravaka, T., Shaista Sheila, H.S.L., Rakotomavojaona, T., Rakoto-Alson, A.O. and Rasamindrakotroka, A. (2015) Changing Profile and Increasing Antimicrobial Resistance of Uropathogenic Bacteria in Madagascar. Médecine et Maladies Infectieuses, 45, 173-176. https://doi.org/10.1016/j.medmal.2015.03.006
[23]
Zalegh, I., Chaoui, L., Maaloum, F., Zerouali, K. and Mhand, R.A. (2023) Prevalence of Multidrug-Resistant and Extensively Drug-Resistant Phenotypes of Gram-Negative Bacilli Isolated in Clinical Specimens at Centre Hospitalo-Universitaire IBN Rochd, Morocco. Pan-African Medical Journal, 45, Article No. 41.
[24]
Bhargava, K., Nath, G., Bhargava, A., Kumari, R., Aseri, G.K. and Jain, N. (2022) Bacterial Profile and Antibiotic Susceptibility Pattern of Uropathogens Causing Urinary Tract Infection in the Eastern Part of Northern India. Frontiers in Microbiology, 13, Article ID: 965053. https://doi.org/10.3389/fmicb.2022.965053
[25]
Djim-Adjim-Ngana, K., Oumar, L.A., Mbiakop, B.W., Munshili Njifon, H.L., Crucitti, T., Nchiwan, E.N., et al. (2020) Prevalence of Extended-Spectrum Beta-Lactamase-Producing Enterobacterial Urinary Infections and Associated Risk Factors in Small Children of Garoua, Northern Cameroon. Pan African Medical Journal, 36, 157-167. https://doi.org/10.11604/pamj.2020.36.157.21347
[26]
Modukuru, G.K., Surya, P.M.S., Kakumanu, V.R. and Yarava, S. (2021) Phenotypic Characterization of Macrolide-Lincosamide-Streptogramin B Resistance in Staphylococcus aureus. Journal of Pure and Applied Microbiology, 15, 689-694. https://doi.org/10.22207/jpam.15.2.18
