%0 Journal Article
%T Phenotypic Resistance of Bacteria Isolated from Urinary Tract Infections at the Protestant Hospital of Ngaoundere (Cameroon)
%A Benjamin Tangue Talom
%A Berinyuy Moniratou
%A Simeon Pierre Chegaing Fodouop
%A Michel Archange Tagne Fokam
%A Carolle Sylvie Dongmo Meffo
%A Zelda In&#232
%A s Eguen
%A Jules-Roger Kuiate
%J Journal of Biosciences and Medicines
%P 243-254
%@ 2327-509X
%D 2025
%I Scientific Research Publishing
%R 10.4236/jbm.2025.131020
%X <b>Aims</b> <b>and</b> <b>objectives:</b> The frequent and unprescribed use of antibiotics has led to the development of resistance by microorganisms responsible for urinary tract infection (UTI). In order to facilitate the follow-up of this microbial resistance, the aim of this study was to determine the bacteria resistant phenotypes. <b>Method:</b> To achieve the following objectives, this study was conducted from June to August 2023. The isolation and identification were performed by standard methods why susceptibility testing was done by Kirby-Bauer disk diffusion technique according to CLSI guidelines. Double-disk synergy test was applied to determine the presence of extended-spectrum <i>&#946;</i>-lactamase (ESBL) produced by bacteria. The Imipenem EDTA Combined Disc Test (CDT) for Metallo beta lactamase (MBL) screening, the D-zone test to detect macrolides, lincosamides and streptogramins type B (MLS<sub>B</sub>) and Meticillin resistant <i>Staphy</i><i>lococcus</i> <i>aureus</i> (MRS A) which was assessed using a Cefoxitin (30 &#181;g) disc. <b>Results:</b> A total of 40 bacteria were identified with a prevalence of 37.03%. Overall, <i>E.</i> <i>coli</i> was the predominant isolate 14 (35%), followed by <i>Staphylococcus</i> <i>aureus</i> 10 (25%) and <i>Klesbsiella</i> <i>pneumonia</i> 4 (10%). <i>Pseudomonas</i> <i>aeruginosa,</i> <i>Salmonella</i> <i>arinosa</i> and <i>Enterobacter</i> were the most sensible (100%) bacteria against ciprofloxin, ceftriaxone and cefixime. Almost all bacteria were resistant to Amoxicillin/clavulanic acid (&gt;50%). The isolates were in the majority resistant to imipenem. ESBL-producing Enterobacteriaceae represented 25.92%, with a higher rate among <i>E.</i> <i>coli</i>. No MBL production was found among the isolates while 38.46% represented cMLS<sub>B</sub>, 15.38% represented iMLS<sub>B</sub>, 23.07% represented MS<sub>B</sub> and 23.07% represented MRSA. <b>Conclusion:</b> Clinical strains of UTI from Protestant Hospital of Ngaoundere exhibiting ESBL, cMLS<sub>B</sub>, iMLS<sub>B</sub>, MS<sub>B</sub> and MRSA. The regular updating of antibiotic resistance statistics of isolated strains allows for a better adaptation of probabilistic antibiotic therapy.
%K Enterobacteriaceae
%K Resistance Profile
%K Phenotypic Detection
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=140071