|
|
探究一款后生元牙膏对口腔菌群的影响
|
Abstract:
目的：探究一款后生元牙膏对健康人群口腔菌群的影响。方法：纳入30名口腔健康人群，在使用牙膏前(0 d)和使用30天后(30 d)分别采集唾液，测定PLI值和CAT值，并进行16S rDNA测序，分析两组菌群结构及多样性差异。结果：与使用前相比，使用牙膏后的PLI值和CAT值均显著降低(P < 0.0001)。使用牙膏前后口腔菌群的优势菌基本一致，但在相对丰度上存在差异。与使用前相比，使用牙膏后的Alpha多样性指数Ace和Chao均显著降低(P < 0.0001)。PCA分析结果显示，使用牙膏前后的口腔菌群组成差异主要由嗜二氧化碳细胞菌属(Capnocytophaga)和孪生球菌属(Gemella)导致。结论：该款后生元牙膏能够有效减少牙菌斑，降低龋病风险，并能够调节口腔微生态。
Objective: To investigate the effect of a postbiotic toothpaste on the oral microbiota of healthy people. Methods: A total of 30 oral health individuals were enrolled, and saliva was collected before (0 day) and 30 days after 30 days (30 days) of toothpaste, and PLI and CAT values were measured, and 16S rDNA sequencing was performed to analyze the differences in microbial community structure and diversity between the two groups. Results: Compared with the before use, the PLI value and CAT value were significantly reduced after using toothpaste (P < 0.0001). The dominant bacteria in the oral microbiota were basically the same before and after the use of toothpaste, but there were differences in relative abundance. Compared with the before use, the alpha diversity index Ace and Chao were significantly decreased after using toothpaste (P < 0.0001). The results of PCA analysis showed that the differences in oral microbiota composition before and after toothpaste use were mainly caused by Capnocytophaga and Gemella. Conclusion: This postbiotic toothpaste can effectively reduce dental plaque, reduce the risk of caries, and regulate the oral microecology.
| [1] | 陈敏珊, 郑娟, 李平. 口腔微生态的研究进展及益生菌在口腔护理中的应用研究[J]. 口腔护理用品工业, 2023, 33(1): 4-12. |
| [2] | 杨洁如, 吕文飞, 胡欢, 等. 氟在口腔微生态作用中的研究进展[J]. 微生物学通报, 2024, 6(11): 1-11 |
| [3] | Moran, G.P., Zgaga, L., Daly, B., Harding, M. and Montgomery, T. (2023) Does Fluoride Exposure Impact on the Human Microbiome? Toxicology Letters, 379, 11-19. https://doi.org/10.1016/j.toxlet.2023.03.001 |
| [4] | 胡佩男, 刘洋, 李阳, 等. 口腔微生态与龋病防治的研究进展[J]. 现代医药卫生, 2022, 38(9): 1531-1535. |
| [5] | 张玉英. 口腔微生态PH平衡与龋病牙周炎相关性研究[D]: [硕士学位论文]. 青岛: 青岛大学, 2016. |
| [6] | de Mendoza, I.L.I., Mendia, X.M., de la Fuente, A.M.G., Andrés, G.Q. and Urizar, J.M.A. (2019) Role of porphyromonas gingivalis in Oral Squamous Cell Carcinoma Development: A Systematic Review. Journal of Periodontal Research, 55, 13-22. https://doi.org/10.1111/jre.12691 |
| [7] | 骆凯华, 彭显, 李继遥. 牙周致病菌促进心血管疾病发生发展的分子机制研究进展[J]. 口腔疾病防治, 2023, 31(2): 147-152. |
| [8] | Ruff, W.E., Greiling, T.M. and Kriegel, M.A. (2020) Host-Microbiota Interactions in Immune-Mediated Diseases. Nature Reviews Microbiology, 18, 521-538. https://doi.org/10.1038/s41579-020-0367-2 |
| [9] | 刘蓉, 杨琨. 牙周炎通过菌群作用促进类风湿关节炎的研究进展[J]. 口腔医学研究, 2023, 39(5): 392-395. |
| [10] | 陈馨怡, 姚运海, 张涵清. 口腔微生态与人类疾病发生的研究[J]. 系统医学, 2023, 8(10): 190-193. |
| [11] | 陆艺峰, 戴振刚. 益生菌牙膏与抗菌测试[J]. 口腔护理用品工业, 2021, 31(6): 20-22. |
| [12] | 陈健芬, 宋云云, 孙东方, 等. 可量化的益生菌与茶多酚复配模型的抑菌机理及其在牙膏中的功效研究[J]. 口腔护理用品工业, 2022, 32(4): 4-11. |
| [13] | 王冬雪, 张倩霞, 文艺, 等. 益生元对口腔健康促进作用和机制研究进展[J]. 微生物学通报, 2023, 50(2): 742-753. |
| [14] | Li, X., Zhong, Y., Jiang, X., et al. (2015) Randomized Clinical Trial of the Efficacy of Dentifrices Containing 1.5% Arginine, an Insoluble Calcium Compound and 1450 ppm Fluoride Over Two Years. The Journal of Clinical Dentistry, 26, 7-12. |
| [15] | Leila, B., Eskandar, M., Afrooz, S., et al. (2022) Effect of Postbiotic-Toothpaste on Salivary Levels of IgA in 6-to 12-Year-Old Children: Study Protocol for a Randomized Triple-Blind Placebo-Controlled Trial. Frontiers in Pediatrics, 10, Article 1042973. https://doi.org/10.3389/fped.2022.1042973 |
| [16] | 林祯灏, 李素洁. 益生菌联合牙周基础治疗对慢性牙周炎患者牙龈出血指数及牙齿松动度的影响[J]. 临床医学工程, 2022, 29(4): 481-482. |
| [17] | 赵素倩, 穆楠楠, 赵大敏, 等. 益生菌联合新癀片治疗牙周病的效果分析[J]. 南昌大学学报(医学版), 2023, 63(3): 60-63, 92. |
| [18] | Zanatta, C.A.R., Fritz, P.C., Comelli, E.M. and Ward, W.E. (2021) Intervention with Inulin Prior to and during Sanative Therapy to Further Support Periodontal Health: Study Protocol for a Randomized Controlled Trial. Trials, 22, Article No. 527. https://doi.org/10.1186/s13063-021-05504-1 |
| [19] | 刘清. 基于16S rRNA全长测序的后生元含片辅助龋病充填治疗人群的口腔菌群分析[D]: [硕士学位论文]. 南昌: 南昌大学, 2022. |
| [20] | Luo, S., Wei, S., Luo, X., Yang, Q., Wong, K., Cheung, P.C.K., et al. (2024) How Probiotics, Prebiotics, Synbiotics, and Postbiotics Prevent Dental Caries: An Oral Microbiota Perspective. npj Biofilms and Microbiomes, 10, Article No. 14. https://doi.org/10.1038/s41522-024-00488-7 |
| [21] | 赵琛, 华红, 闫志敏. 益生菌预防和治疗口腔疾病的研究进展[J]. 口腔医学研究, 2016, 32(4): 418-420. |
| [22] | Salminen, S., Collado, M.C., Endo, A., Hill, C., Lebeer, S., Quigley, E.M.M., et al. (2021) The International Scientific Association of Probiotics and Prebiotics (ISAPP) Consensus Statement on the Definition and Scope of Postbiotics. Nature Reviews Gastroenterology & Hepatology, 18, 649-667. https://doi.org/10.1038/s41575-021-00440-6 |
| [23] | Moradi, M., Kousheh, S.A., Almasi, H., Alizadeh, A., Guimarães, J.T., Yılmaz, N., et al. (2020) Postbiotics Produced by Lactic Acid Bacteria: The Next Frontier in Food Safety. Comprehensive Reviews in Food Science and Food Safety, 19, 3390-3415. https://doi.org/10.1111/1541-4337.12613 |
| [24] | 王扬蕊, 史晓丹, 杨雨宁, 等. 后生元制备技术及益生效应研究进展[J]. 食品与生物技术学报, 2024, 43(4): 8-16. |
| [25] | 牙膏功效评价(WS/T 326-2010) [J]. 口腔护理用品工业, 2010, 20(6): 11-15. |
| [26] | Macgregor, I.D. (1987) Comparison of the Silness-Loe (1964) Index with Gravimetric Measurement of Dental Plaque. Clinical Preventive Dentistry, 9, 9-12. |
| [27] | Koroluk, L., Hoover, J.N. and Komiyama, K. (1994) The Sensitivity and Specificity of a Colorimetric Microbiological Caries Activity Test (Cariostat) in Preschool Children. Pediatric Dentistry, 16, 276-281. |
| [28] | Watts, G.S., Youens‐Clark, K., Slepian, M.J., Wolk, D.M., Oshiro, M.M., Metzger, G.S., et al. (2017) 16S rRNA Gene Sequencing on a Benchtop Sequencer: Accuracy for Identification of Clinically Important Bacteria. Journal of Applied Microbiology, 123, 1584-1596. https://doi.org/10.1111/jam.13590 |
| [29] | Iglesias, R.A., González, V.L., Castro, B.C., et al. (2024) Impact of 16S rRNA Gene Redundancy and Primer Pair Selection on the Quantification and Classification of Oral Microbiota in Next-Generation Sequencing. Microbiology Spectrum, 11, e0439822. https://doi.org/10.1128/spectrum.04398-22 |
| [30] | Salminen, S., Collado, M.C., Endo, A., Hill, C., Lebeer, S., Quigley, E.M.M., et al. (2021) The International Scientific Association of Probiotics and Prebiotics (ISAPP) Consensus Statement on the Definition and Scope of Postbiotics. Nature Reviews Gastroenterology & Hepatology, 18, 649-667. https://doi.org/10.1038/s41575-021-00440-6 |
| [31] | Holz, C., Alexander, C., Balcke, C., Moré, M., Auinger, A., Bauer, M., et al. (2013) Lactobacillus paracasei DSMZ16671 Reduces Mutans Streptococci: A Short-Term Pilot Study. Probiotics and Antimicrobial Proteins, 5, 259-263. https://doi.org/10.1007/s12602-013-9148-9 |
| [32] | Lin, W., Kuo, Y., Chen, C., Huang, Y., Hsu, C., Lin, J., et al. (2021) Viable and Heat-Killed Probiotic Strains Improve Oral Immunity by Elevating the Iga Concentration in the Oral Mucosa. Current Microbiology, 78, 3541-3549. https://doi.org/10.1007/s00284-021-02569-8 |
| [33] | 王春梅, 李琳. 减轻口臭牙膏的研发[J]. 口腔护理用品工业, 2023, 33(6): 33-36. |
| [34] | Sabharwal, A., Ganley, K., Miecznikowski, J.C., Haase, E.M., Barnes, V. and Scannapieco, F.A. (2018) The Salivary Microbiome of Diabetic and Non‐Diabetic Adults with Periodontal Disease. Journal of Periodontology, 90, 26-34. https://doi.org/10.1002/jper.18-0167 |
| [35] | Zhu, W., Shen, W., Wang, J., Xu, Y., Zhai, R., Zhang, J., et al. (2022) Capnocytophaga gingivalis Is a Potential Tumor Promotor in Oral Cancer. Oral Diseases, 30, 353-362. https://doi.org/10.1111/odi.14376 |
| [36] | Kotrashetti, V., Idate, U., Bhat, K., Kugaji, M. and Kumbar, V. (2020) Molecular Identification of Capnocytophaga Species from the Oral Cavity of Patients with Chronic Periodontitis and Healthy Individuals. Journal of Oral and Maxillofacial Pathology, 24, 397. https://doi.org/10.4103/jomfp.jomfp_33_20 |
| [37] | Ogunkolade, W., Senusi, A.A., Desai, P., Sacoor, S., Bibi, A., Gokani, B., et al. (2023) Profiling the Microbiome of Oral and Genital Mucosal Surfaces in Behçet’s Disease. Clinical Immunology, 253, Article 109654. https://doi.org/10.1016/j.clim.2023.109654 |
| [38] | McDaniel, J., McDaniel, S., Samiano, B.J., Marrujo, M., Kingsley, K. and Howard, K.M. (2021) Microbial Screening Reveals Oral Site-Specific Locations of the Periodontal Pathogen Selenomonas noxia. Current Issues in Molecular Biology, 43, 353-364. https://doi.org/10.3390/cimb43010029 |
| [39] | Pignatelli, P., Curia, M.C., Tenore, G., Bondi, D., Piattelli, A. and Romeo, U. (2024) Oral Bacteriome and Oral Potentially Malignant Disorders: A Systematic Review of the Associations. Archives of Oral Biology, 160, Article 105891. https://doi.org/10.1016/j.archoralbio.2024.105891 |
