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2014~2023年全球国际高危克隆大肠埃希菌ST131的群体遗传特征研究
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Abstract:
背景：国际高危克隆大肠埃希菌(Escherichia coli) ST131是重要的医院获得性病原体，会导致尿路感染，血流感染以及呼吸道感染等，甚至死亡，给全球健康带来重大威胁。目的：探究ST131菌株的群体遗传特征，为控制大肠埃希菌抗生素耐药问题提供更多见解。结果：本研究收集2014~2023年来自全球的7735株ST131菌株全基因组测序数据，对其基因组特征进行了分析发现sul1 (54.6%)、mph(A) (53.3%)、tet(A) (52.5%)、dfrA17 (49.4%)、sul2 (38.4%)、aph(3'')-Ib (38.0%)，aph(6)-Id (37.6%)，blaTEM-1B (37.2%)，blaCTX-M-15 (37.2%)是全球ST131菌株的优势磺胺类、大环内酯类、四环素类、甲氧苄啶类、氨基糖苷类、β-内酰胺类抗生素耐药基因(ARGs)变体。blaCTX-M-27、blaNDM-5、blaKPC-2、blaKPC-3的检出率随时间呈升高趋势；而aac(3)-IId、aac(6')-Ib-cr、blaOXA-1、catB3的检出呈下降趋势；同时aadA5、aph(3'')-Ib、aph(6)-Id、blaTEM-1B、dfrA17、mph(A)、tet(A)的检出呈波动变化(先升高再降低)。O25:H4是ST131的优势血清型。共检出543种毒力基因(VGs)，与菌株粘附、定植等功能有关。抗生素耐药基因(ARGs)和毒力基因(VGs)的数量随时间增加而增多(P < 0.01)。结论：ST131大肠埃希菌在全球持续传播，菌株向着高耐药和高毒力的方向进化，正在对临床治疗造成重大威胁。
Background: The international high-risk clone Escherichia coli (ST131) is an important hospital-acquired pathogen that causes urinary tract infections, bloodstream infections, and respiratory infections, among other things, and even death, posing a major threat to global health. Objective: To explore the population genetic characteristics of ST131 strains and provide more insights into the problem of controlling antibiotic resistance in Escherichia coli. Results: In this study, we collected whole genome sequencing data of 7735 ST131 strains from around the world from 2014 to 2023 and analysed their genomic characteristics, found that sul1 (54.6%), mph(A) (53.3%), tet(A) (52.5%), dfrA17 (49.4%), sul2 (38.4%), aph(3'')-Ib (38.0%), aph(6)-Id (37.6%), blaTEM-1B (37.2%), blaCTX-M-15 (37.2%) are the dominant antibiotic-resistant gene(ARGs) variants of sulfonamides, macrolides, tetracyclines, mephedrone, aminoglycosides, and β-lactams for the global ST131 strain. blaCTX-M-27, blaNDM-5,
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