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肺腺癌中3P_tRNA-Arg-TCT-4-1的表达及对增殖的影响
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Abstract:
目的：研究肺腺癌(LUAC)中3P_tRNA-Arg-TCT-4-1的表达情况及其临床意义，并探索3P_tRNA-Arg-TCT-4-1对LUAC细胞生长和和生存预后的影响。方法：收集我院手术切除的LUAC 71例标本及临床特征。qRT-PCR检测3P_tRNA-Arg-TCT-4-1在LUAC组织中的表达水平，统计分析3P_tRNA-Arg-TCT-4-1的表达与LUAC病理特征的相关性。通过Kaplan-Meier统计方法分析3P_tRNA-Arg-TCT-4-1表达对肺腺癌患者生存预后的影响。采用CCK-8增殖实验检测3P_tRNA-Arg-TCT-4-1对LUAC细胞增殖的影响。结果：qRT-PCR实验结果显示，在71对LUAC组织中3P_tRNA-Arg-TCT-4-1相对表达量为3.491 ± 0.222，明显高于癌旁正常组织(t = 10.064, P = 0.000 < 0.05)；统计分析结果显示3P_tRNA-Arg-TCT-4-1的表达与LUAC的分化程度(χ2 = 11.112, P = 0.001)和肿瘤大小(χ2 = 4.616, P = 0.032)密切相关(P均 < 0.05)，而与患者年龄、性别、淋巴结转移和TNM分期之间无相关性(P均 > 0.05)。在与患者生存预后的关系上，3P_tRNA-Arg-TCT-4-1正常或阴性表达组总生存期时间明显长于高表达组(χ2 = 5.885, P = 0.002 < 0.05)。CCK-8增殖实验提示过表达3P_tRNA-Arg-TCT-4-1增强了LUCA细胞的增殖活性，而降低3P_tRNA-Arg-TCT-4-1表达则减弱了LUCA细胞的增殖活性(P均 < 0.05)。结论：在LUCA中3P_tRNA-Arg-TCT-4-1表达水平升高，是一个新的促进增殖的促癌基因；3P_tRNA-Arg-TCT-4-1有可能成为治疗生长旺盛的LUCA分子标志物。
Objective: To investigate the expression of 3P_tRNA-Arg-TCT-4-1 in lung adenocarcinoma (LUAC) and its clinical significance, and to explore the effect of 3P_tRNA-Arg-TCT-4-1 on the growth, survival and prognosis of LUAC cells. Methods: The clinical features and specimens of 71 pairs of LUAC were collected. The expression level of 3P_tRNA-Arg-TCT-4-1 in LUAC tissues was detected by qRT-PCR, and the correlation between the expression of 3P_tRNA-Arg-TCT-4-1 and the pathological characteristics of LUAC was statistically analyzed. The effect of 3P_tRNA-Arg-TCT-4-1 expression on survival and prognosis of lung adenocarcinoma patients was analyzed by the Kaplan-Meier statistical method. The effect of 3P_tRNA-Arg-TCT-4-1 on the proliferation of LUAC cells was detected by the CCK-8 proliferation assay. Results: The results of qRT-PCR showed that the relative expression of 3P_tRNA-Arg-TCT-4-1 in 71 pairs of LUAC tissues was 3.491 ± 0.222, which was significantly higher than that in adjacent normal tissues (t = 10.064, P = 0.000 < 0.05). Statistical analysis showed that the expression of 3P_tRNA-Arg-TCT-4-1 was closely correlated with the differentiation degree of LUAC (χ2 = 11.112, P = 0.001) and tumor size (χ2 = 4.616, P = 0.032) (both P < 0.05). There was no correlation with age, sex, lymph node metastasis, and TNM stage (all P > 0.05). With survival and prognosis, the total survival time of the 3P_tRNA-Arg-TCT-4-1 expression group was significantly longer than that of the high expression group (χ2 = 5.885, P = 0.002 <
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