%0 Journal Article
%T 肺腺癌中3P_tRNA-Arg-TCT-4-1的表达及对增殖的影响<br>Expression of 3P_tRNA-Arg-TCT-4-1 in Lung Adenocarcinoma and Its Effect on Proliferation
%A 何志鹤
%A 王成海
%J Advances in Clinical Medicine
%P 588-595
%@ 2161-8720
%D 2024
%I Hans Publishing
%R 10.12677/acm.2024.1472055
%X 目的：研究肺腺癌(LUAC)中3P_tRNA-Arg-TCT-4-1的表达情况及其临床意义，并探索3P_tRNA-Arg-TCT-4-1对LUAC细胞生长和和生存预后的影响。方法：收集我院手术切除的LUAC 71例标本及临床特征。qRT-PCR检测3P_tRNA-Arg-TCT-4-1在LUAC组织中的表达水平，统计分析3P_tRNA-Arg-TCT-4-1的表达与LUAC病理特征的相关性。通过Kaplan-Meier统计方法分析3P_tRNA-Arg-TCT-4-1表达对肺腺癌患者生存预后的影响。采用CCK-8增殖实验检测3P_tRNA-Arg-TCT-4-1对LUAC细胞增殖的影响。结果：qRT-PCR实验结果显示，在71对LUAC组织中3P_tRNA-Arg-TCT-4-1相对表达量为3.491 ± 0.222，明显高于癌旁正常组织(t = 10.064, <i>P</i> = 0.000 &lt; 0.05)；统计分析结果显示3P_tRNA-Arg-TCT-4-1的表达与LUAC的分化程度(<i>χ</i><sup>2</sup> = 11.112, <i>P</i> = 0.001)和肿瘤大小(<i>χ</i><sup>2</sup> = 4.616, <i>P</i> = 0.032)密切相关(<i>P</i>均 &lt; 0.05)，而与患者年龄、性别、淋巴结转移和TNM分期之间无相关性(<i>P</i>均 &gt; 0.05)。在与患者生存预后的关系上，3P_tRNA-Arg-TCT-4-1正常或阴性表达组总生存期时间明显长于高表达组(<i>χ</i><sup>2</sup> = 5.885, <i>P</i> = 0.002 &lt; 0.05)。CCK-8增殖实验提示过表达3P_tRNA-Arg-TCT-4-1增强了LUCA细胞的增殖活性，而降低3P_tRNA-Arg-TCT-4-1表达则减弱了LUCA细胞的增殖活性(<i>P</i>均 &lt; 0.05)。结论：在LUCA中3P_tRNA-Arg-TCT-4-1表达水平升高，是一个新的促进增殖的促癌基因；3P_tRNA-Arg-TCT-4-1有可能成为治疗生长旺盛的LUCA分子标志物。<br />
Objective: To investigate the expression of 3P_tRNA-Arg-TCT-4-1 in lung adenocarcinoma (LUAC) and its clinical significance, and to explore the effect of 3P_tRNA-Arg-TCT-4-1 on the growth, survival and prognosis of LUAC cells. Methods: The clinical features and specimens of 71 pairs of LUAC were collected. The expression level of 3P_tRNA-Arg-TCT-4-1 in LUAC tissues was detected by qRT-PCR, and the correlation between the expression of 3P_tRNA-Arg-TCT-4-1 and the pathological characteristics of LUAC was statistically analyzed. The effect of 3P_tRNA-Arg-TCT-4-1 expression on survival and prognosis of lung adenocarcinoma patients was analyzed by the Kaplan-Meier statistical method. The effect of 3P_tRNA-Arg-TCT-4-1 on the proliferation of LUAC cells was detected by the CCK-8 proliferation assay. Results: The results of qRT-PCR showed that the relative expression of 3P_tRNA-Arg-TCT-4-1 in 71 pairs of LUAC tissues was 3.491 ± 0.222, which was significantly higher than that in adjacent normal tissues (t = 10.064, <i>P</i> = 0.000 &lt; 0.05). Statistical analysis showed that the expression of 3P_tRNA-Arg-TCT-4-1 was closely correlated with the differentiation degree of LUAC (<i>χ</i><sup>2</sup> = 11.112, <i>P</i> = 0.001) and tumor size (<i>χ</i><sup>2</sup> = 4.616, <i>P</i> = 0.032) (both <i>P</i> &lt; 0.05). There was no correlation with age, sex, lymph node metastasis, and TNM stage (all <i>P </i>&gt; 0.05). With survival and prognosis, the total survival time of the 3P_tRNA-Arg-TCT-4-1 expression group was significantly longer than that of the high expression group (<i>χ</i><sup>2</sup> = 5.885, <i>P</i> = 0.002 &lt;
%K 肺腺癌，3P_tRNA-Arg-TCT-4-1，增殖活性，临床特征<br>Lung Adenocarcinoma
%K 3P_tRNA-Arg-TCT-4-1
%K Proliferative Activity
%K Clinical Features
%U http://www.hanspub.org/journal/PaperInformation.aspx?PaperID=91744