In this work, AgCl nanoparticles were synthesized from Microsorum scolopendria (MS) aqueous extract and AgNO3 solution. Preliminary confirmation was a color change from a light brown to a dark-colored solution and a UV-Vis spectra surface plasmon resonance peak at 427 nm. Measured vibrational frequencies at 1713 cm−1 and 1030 cm−1 for C-O stretching of carboxylic acid or aliphatic ketone, and 1547 cm−1 for possibly N-O stretching of nitro compounds by Infrared (FTIR) analysis explain the possible biomaterial electronegative species or functional groups responsible for the reduction of Ag ( 1) to Ag (0) for the formation of MS-AgCl nanoparticles. XRD analysis studies revealed that these particles contained face-centered cubic crystallites of metallic AgCl of 100 % with an average calculated crystallite size range of 30.34 nm (SD = 5.10 nm) by Scherrer’s equation and a calculated crystallite size of 66.04 nm with a lattice strain of 0.00175 nm by Williamson Hall equation. The measured albumin denaturing activity of MS-AgCl nanoparticles gave an IC50 value of 26.70 µg/mL and 1.35 µg/mL for the positive control diclofenac. Additionally, the measured ability of phosphomolybdate complex formation, the antioxidant IC₅₀ value of MS-AgCl nanoparticles was 35.29 µg/mL, and positive control ascorbic acid was 13.91 µg/mL. In all, using MS fern frond aqueous extracts, this preliminary work confirms MS-AgCl nanoparticles as potential therapeutic agents for oxidative stress, inflammatory problems, and related diseases.
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