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基于荧光法的DNA浓度测量方法研究
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Abstract:
DNA浓度测量在分子生物学中具有重要的应用价值。传统的基于吸光度法测量灵敏度低，需要试剂量大，为解决该问题，本文构建了基于荧光检测的高灵敏度微型DNA浓度测量系统。选取光源为中心波长505 nm激光模组，通过将SYBR Green I染料与DNA混合，研究了荧光强度与DNA浓度之间的量化关系。结果表明，DNA浓度在1~16 ng/μL范围内，与其荧光强度呈线性关系，相关系统高达0.994。本研究为小型DNA浓度测量系统的开发奠定了实验基础。
DNA concentration measurement has important application value in molecular biology. The traditional absorbance-based measurement has low sensitivity and requires a large amount of reagents, in order to solve this problem, this paper constructed a highly sensitive micro DNA concentration measurement system based on fluorescence detection. The light source was selected as a laser module with a center wavelength of 505 nm, and the quantitative relationship between fluorescence intensity and DNA concentration was investigated by mixing SYBR Green I dye with DNA. The results showed that the DNA concentration was linearly related to its fluorescence intensity in the range of 1~16 ng/μL, and the correlation system was as high as 0.994. This study lays an experimental foundation for the development of a miniature DNA concentration measurement system.
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