%0 Journal Article
%T 基于荧光法的DNA浓度测量方法研究<br>Measurement of DNA Concentration Based on Fluorescence
%A 张振
%A 陈沁
%A 刘张桃
%A 曾媛
%A 李振庆
%J Advances in Analytical Chemistry
%P 55-60
%@ 2163-1565
%D 2024
%I Hans Publishing
%R 10.12677/aac.2024.142007
%X DNA浓度测量在分子生物学中具有重要的应用价值。传统的基于吸光度法测量灵敏度低，需要试剂量大，为解决该问题，本文构建了基于荧光检测的高灵敏度微型DNA浓度测量系统。选取光源为中心波长505 nm激光模组，通过将SYBR Green I染料与DNA混合，研究了荧光强度与DNA浓度之间的量化关系。结果表明，DNA浓度在1~16 ng/μL范围内，与其荧光强度呈线性关系，相关系统高达0.994。本研究为小型DNA浓度测量系统的开发奠定了实验基础。<br />
DNA concentration measurement has important application value in molecular biology. The traditional absorbance-based measurement has low sensitivity and requires a large amount of reagents, in order to solve this problem, this paper constructed a highly sensitive micro DNA concentration measurement system based on fluorescence detection. The light source was selected as a laser module with a center wavelength of 505 nm, and the quantitative relationship between fluorescence intensity and DNA concentration was investigated by mixing SYBR Green I dye with DNA. The results showed that the DNA concentration was linearly related to its fluorescence intensity in the range of 1~16 ng/μL, and the correlation system was as high as 0.994. This study lays an experimental foundation for the development of a miniature DNA concentration measurement system.
%K DNA，荧光染料，浓度测量<br>DNA
%K Fluorescent Dye
%K Concentration Measurement
%U http://www.hanspub.org/journal/PaperInformation.aspx?PaperID=86366