高效液相色谱法同时测定跌打活血散中羟基红花黄色素A和阿魏酸的含量
Simultaneous Determination of Hydroxysafflor Yellow A and Ferulic Acid in Diedahuoxue Powder by HPLC

目的：建立跌打活血散中红花和当归的主要成分羟基红花黄色素A和阿魏酸的含量测定方法。方法：以Agilent ZORBAXSB-C8柱(5 μm, 250 mm × 4.6 mm)为色谱柱，乙腈-0.1%磷酸-四氢呋喃(18:80:2)为流动相，检测波长为340 nm，流速为1.0 ml/min，柱温为30℃。结果：羟基红花黄色素A在40.2~201.0 μg/mL浓度范围内线形关系良好，r = 0.9995 (RSD = 1.6%, n = 5)，平均回收率为96.2% (RSD = 1.3%, n = 6)；阿魏酸在1.01~5.05 μg/mL浓度范围内线形关系良好，r = 0.9997 (RSD = 1.1%, n = 5)，平均回收率为98.6% (RSD = 1.5%, n = 6)。结论：本方法简便、准确，结果稳定，可用于跌打活血散中红花和当归的主要成分羟基红花黄色素A和阿魏酸的测定。
Object: To establish a method for the determination of Hydroxysafflor yellow A and ferulic acid in diedahuoxue powder. Methods: Agilent ZORBAX SB-C8 column (5 μm, 250 mm × 4.6 mm) was used as the chromatographic column, acetonitrile-0.1% phosphoric acid-tetrahydrofuran (18:80:2) as the mobile phase, the detection wavelength was 340 nm, the flow rate was 1.0 ml/min, and the column temperature was 30？C. Results: The linear range of Hydroxysafflor yellow A was 40.2~201.0 μg/ml, r = 0.9995 (RSD = 1.6%, n = 5), the average recovery was 96.2% (RSD = 1.3%, n = 6); the linear range of Ferulic Acid was 1.01~5.05 μg/ml, r = 0.9997 (RSD = 1.1%, n = 5), the average recovery was 98.6% (RSD = 1.5%, n = 6). Conclusion: The method is simple, accurate and stable. It can be used for the determination of Hydroxysafflor yellow A and Ferulic Acid in diedahuoxue Powder.