Seroprevalence of HBV and HCV among People Living with HIV in Burkina Faso and Diagnostic Performance of HIV/HCV/HBsAg Combined Rapid Test in Comparison with Architect Assays
Background: The diagnosis of
human immunodeficiency virus (HIV), hepatitis B virus (HBV), and hepatitis C
virus (HCV) remains a constraint for some populations in sub-Saharan Africa.
This study aimed to determine the prevalence of HBV and HCV in people living
with HIV and to evaluate the performance of a combined rapid test for the
simultaneous detection of HIV, HBV, and HCV. Methods: This is a
cross-sectional study that took place from February 2017 to November 2018 and
included 139 HIV-infected individuals followedup at different medical centers in Ouagadougou, Burkina Faso. HBV and HCV
serology tests were performed on-site using finger prick whole blood with
HIV/HCV/HBsAg combined rapid test and then serum with two reference tests
“Architect HBsAg Qualitative” and “Architect HIV Ag/Ab Combo”. Results:
The mean age of the participants was 57 ± 8 years. Of the 139 participants, 10%
(14/139) were HIV-1 positive, 71.9% (100/139) were HIV-2 positive, and 18.0%
(25/139) were HIV-1/HIV-2 coinfected. The sensitivity and specificity of the
HIV/HCV/HBsAg combined rapid test were 33.33% vs 99.11% and 20% vs 99.25%
compared to Architect HBsAg Qualitative and Architect HIV Ag/Ab Combo,
respectively. The Kappa and Youden Index values were 0.4262 and 0.3244 and
0.2707 and 0.1925, respectively, compared to each of the two reference tests. Conclusion:
The results show that the HIV/HCV/HBsAg combined rapid test has poor diagnostic
efficiency and should not be recommended for the diagnosis of these viruses.
References
[1]
Pant Pai, N. and Daher, J. (2015) Multiplexed Testing for HIV and Related Bacterial and Viral Coinfections at the Point-of-Care: Quo Vadis? Expert Review of Molecular Diagnostics, 15, 463-469. https://doi.org/10.1586/14737159.2015.1021335
[2]
Easterbrook, P.J. (2016) Who to Test and How to Test for Chronic Hepatitis C Infection—2016 WHO Testing Guidance for Low- and Middle-Income Countries. Journal of Hepatology, 65, S46-S66. https://doi.org/10.1016/j.jhep.2016.08.002
[3]
WHO (2017) WHO Guidelines on Hepatitis B and C Testing. https://apps.who.int/iris/bitstream/handle/10665/254621/9789241549981-eng.pdf
[4]
Pai, N.P. (2015) Multiplexed Point-of-Care Assays for HIV and Coinfections for Resource Constrained Settings: A Perspective. Future Microbiology, 10, 1393-1396. https://doi.org/10.2217/fmb.15.77
[5]
Soubeiga, S.T., Compaore, R., Djigma, F., Zagre, N., Assengone, E., Traore, L., et al. (2015) Evaluation of Antiretroviral Therapy on Mother to Child Transmission HIV in HIV-1 Positive Pregnant Women: Case of St. Camillus Medical Center in Ouagadougou, Burkina Faso. The Pan African Medical Journal, 20, Article 399. https://doi.org/10.11604/pamj.2015.20.399.5627
[6]
Tang, L.S., Covert, E., Wilson, E. and Kottilil, S. (2018) Chronic Hepatitis B Infection: A Review. JAMA, 319, 1802-1813. https://doi.org/10.1001/jama.2018.3795
[7]
Robin, L., Bouassa, R.S.M., Nodjikouambaye, Z.A., Charmant, L., Matta, M., Simon, S., et al. (2018) Analytical Performances of Simultaneous Detection of HIV-1, HIV-2 and Hepatitis C-Specific Antibodies and Hepatitis B Surface Antigen (HBsAg) by Multiplex Immunochromatographic Rapid Test with Serum Samples: A Cross- Sectional Study. Journal of Virological Methods, 253, 1-4. https://doi.org/10.1016/j.jviromet.2017.12.001
[8]
Yooda, A.P., Soubeiga, S.T., Nebie, K.Y., Diarra, B., Sawadogo, S., Ouattara, A.K., et al. (2018) Impact of Multiplex PCR in Reducing the Risk of Residual Transfusion-Transmitted Human Immunodeficiency and Hepatitis B and C Viruses in Burkina Faso. Mediterranean Journal of Hematology and Infectious Diseases, 10, e2018041. https://doi.org/10.4084/mjhid.2018.041
[9]
Landis, J.R. and Koch, G.G. (1977) The Measurement of Observer Agreement for Categorical Data. Biometrics, 33, 159-174. https://doi.org/10.2307/2529310
[10]
Meda, N., Tuaillon, E., Kania, D., Tiendrebeogo, A., Pisoni, A., Zida, S., et al. (2018) Hepatitis B and C Virus Seroprevalence, Burkina Faso: A Cross-Sectional Study. Bulletin of the World Health Organization, 96, 750-759. https://doi.org/10.2471/BLT.18.208603
[11]
Coffie, P.A., Egger, M., Vinikoor, M.J., Zannou, M., Diero, L., Patassi, A., et al. (2017) Trends in Hepatitis B Virus Testing Practices and Management in HIV Clinics across Sub-Saharan Africa. BMC Infectious Diseases, 17, Article No. 706. https://doi.org/10.1186/s12879-017-2768-z
[12]
Kenfack-Momo, R., Kenmoe, S., Takuissu, G.R., Ebogo-Belobo, J.T., Kengne-Ndé, C., Mbaga, D.S., et al. (2022) Epidemiology of Hepatitis B Virus and/or Hepatitis C Virus Infections among People Living with Human Immunodeficiency Virus in Africa: A Systematic Review and Meta-Analysis. PLOS ONE, 17, e0269250. https://doi.org/10.1371/journal.pone.0269250
[13]
Leumi, S., Bigna, J.J., Amougou, M.A., Ngouo, A., Nyaga, U.F. and Noubiap, J.J. (2020) Global Burden of Hepatitis B Infection in People Living with Human Immunodeficiency Virus: A Systematic Review and Meta-Analysis. Clinical Infectious Diseases, 71, 2799-2806. https://doi.org/10.1093/cid/ciz1170
[14]
Boateng, R., Mutocheluh, M., Dompreh, A., Obiri-Yeboah, D., Odame Anto, E., Owusu, M. and Narkwa, P.W. (2019) Sero-Prevalence of Hepatitis B and C Viral Coinfections among HIV-1 Infected ART-Naive Individuals in Kumasi, Ghana. PLOS ONE, 14, e0215377. https://doi.org/10.1371/journal.pone.0215377
[15]
Acharya, C., Dharel, N. and Sterling, R.K. (2015) Chronic Liver Disease in the Human Immunodeficiency Virus Patient. Clinics in Liver Disease, 19, 1-22. https://doi.org/10.1016/j.cld.2014.09.001
[16]
Farahani, M., Mulinder, H., Farahani, A. and Marlink, R. (2017) Prevalence and Distribution of Non-AIDS Causes of Death among HIV-Infected Individuals Receiving Antiretroviral Therapy: A Systematic Review and Meta-Analysis. International Journal of STD & AIDS, 28, 636-650. https://doi.org/10.1177/0956462416632428
[17]
Rockstroh, J.K. (2006) Influence of Viral Hepatitis on HIV Infection. Journal of Hepatology, 44, S25-S27. https://doi.org/10.1016/j.jhep.2005.11.007
[18]
Annison, L., Hackman, H., Eshun, P.F., Annison, S., Forson, P. and Antwi-Baffour, S. (2022) Seroprevalence and Effect of HBV and HCV Coinfections on the Immuno-Virologic Responses of adult HIV-Infected Persons on Anti-Retroviral Therapy. PLOS ONE, 17, e0278037. https://doi.org/10.1371/journal.pone.0278037
[19]
Tonen-Wolyec, S., Djang’eing’a, R.M., Batina-Agasa, S., Kayembe Tshilumba, C., Muwonga Masidi, J., Hayette, M.P. and Bélec, L. (2021) Self-Testing for HIV, HBV, and HCV Using Finger-Stick Whole-Blood Multiplex Immunochromatographic Rapid Test: A Pilot Feasibility Study in Sub-Saharan Africa. PLOS ONE, 16, e0249701. https://doi.org/10.1371/journal.pone.0249701
[20]
Msomi, N., Naidoo, K., Yende-Zuma, N., Padayatchi, N., Govender, K., Singh, J.A., Salim, A., Quarraisha, A. and Koleka Mlisana, K. (2020) High Incidence and Persistence of Hepatitis B Virus Infection in Individuals Receiving HIV Care in KwaZulu-Natal, South Africa. BMC Infectious Diseases, 20, Article No. 847. https://doi.org/10.1186/s12879-020-05575-6
[21]
Peters, D.H., Garg, A., Bloom, G., Walker, D.G., Brieger, W.R. and Rahman, M.H. (2008) Poverty and Access to Health Care in Developing Countries. Annals of the New York Academy of Sciences, 1136, 161-171. https://doi.org/10.1196/annals.1425.011
[22]
WHO (2016) WHO List of Prequalified in Vitro Diagnostic Products. https://extranet.who.int/prequal/sites/default/files/document_files/231120_prequalified_IVD_product_list.pdf
[23]
Monaghan, T.F., Rahman, S.N., Agudelo, C.W., Wein, A.J., Lazar, J.M., Everaert, K. and Dmochowski, R.R. (2021) Foundational Statistical Principles in Medical Research: Sensitivity, Specificity, Positive Predictive Value, and Negative Predictive Value. Medicina, 57, Article 503. https://doi.org/10.3390/medicina57050503
[24]
Kalla, G.C.M., Voundi, E.V., Angwafo III, F., Bélec, L. and Mbopi-Keou, F.X. (2018) Mass Screening for Hepatitis B and C and HIV in Sub-Saharan Africa. The Lancet Infectious Diseases, 18, 716 https://doi.org/10.1016/S1473-3099(18)30343-8
[25]
Coleman, P. (2006) Detecting Hepatitis B Surface Antigen Mutants. Emerging Infectious Diseases, 12, 198-203. https://doi.org/10.3201/eid1203.050038
