全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

Optimization of LPS-Induced Inflammation Model and Its Feasibility as a Fast Screening Model for Cosmetics

DOI: 10.4236/jcdsa.2024.141006, PP. 84-97

Keywords: Cell Culture, Anti-Inflammatory, Lipopolysaccharide, Keratinocytes, THP-1, Inflammatory Factors

Full-Text   Cite this paper   Add to My Lib

Abstract:

Objectives: The existing inflammatory models are concentrated in relatively complex medical fields, and most of them use a single type of cell, and the induction conditions are not uniform, so the current LPS-induced inflammation model is less conducive to the study of skin inflammation. The aim of this research is to enhance the existing LPS-induced inflammation model and establish a skin inflammation model that is suitable for the swift screening of anti-inflammatory agents in the cosmetics industry. Methods: LPS was used to induce inflammatory responses in KC and THP-1 cells. Enzyme-linked immunosorbent assay (ELISA) was employed to assess the levels of IL-1α, IL-8, and TNF-α in the two cell types, while the DCFH-DA probe was utilized to label the levels of reactive oxygen species (ROS) in both cell types. Results: In KC cells, 10 μg/mL of LPS induced a significant upregulation of IL-8 but did not result in elevated expression of IL-1α. However, at 100 μg/mL of LPS, both IL-8 and IL-1α were highly expressed in KC cells. LPS concentrations ranging from 0.01 to 100 μg/mL failed to stimulate TNF-α production in KC cells but induced a gradient increase in ROS levels. In THP-1 cells, LPS concentrations from 0.01 to 100 μg/mL did not induce IL-1α production but significantly elevated IL-8 and led to a gradient increase in TNF-α and ROS. After treatment with 100 μg/mL of LPS, the cosmetic ingredient Rucika KGM mitigated the elevated levels of IL-1α, IL-8, and ROS in LPS-induced KC cells and IL-8 and ROS in THP-1 cells. Conclusion: This study has successfully developed an application-oriented

References

[1]  Archer, N.K., Jo, J.-H., Lee, S.K., et al. (2019) Injury, Dysbiosis and Filaggrin Deficiency Drive Skin Inflammation via Keratinocyte IL-1α Release. Journal of Allergy and Clinical Immunology, 143, 1426-1443. https://doi.org/10.1016/j.jaci.2018.08.042
[2]  Egawa, G. and Kabashima, K. (2016) Multifactorial Skin Barrier Deficiency and Atopic Dermatitis: Essential Topics to Prevent the Atopic March. Journal of Allergy and Clinical Immunology, 138, 350-358. https://doi.org/10.1016/j.jaci.2016.06.002
[3]  Walana, W., Wang, J., Yabasin, I.B., et al. (2018) IL-8 Analogue CXCL8 (3-72) K11R/ G31P, Modulates LPS-Induced Inflammation via AKT1-NF-kβ and ERK1/2-AP-1 pathways in THP-1 Monocytes. Human Immunology, 79, 809-816.
https://doi.org/10.1016/j.humimm.2018.08.007
[4]  Zhao, D.R., Jiang, Y.S., Sun, J.Y., et al. (2019) Elucidation of The Anti-Inflammatory Effect of VanillinIn Lps-Activated THP-1 Cells. Journal of Food Science, 84, 1920-1928. https://doi.org/10.1111/1750-3841.14693
[5]  Chanput, W., Mes, J., Vreeburg, R.A.M., et al. (2010) Transcription Profiles of LPS-Stimulated THP-1 Monocytes and Macrophages: A Tool to Study Inflammation Modulating Effects of Food-Derived Compounds. Food & Function, 1, 254-261.
https://doi.org/10.1039/c0fo00113a
[6]  Jiang, Y.Y., Tsoi, L.C., Billi, A.C., et.al. (2020) Cytokinocytes: The Diverse Contribution of Keratinocytes to Immune Responses in Skin. JCI Insight, 5, e142067.https://doi.org/10.1172/jci.insight.142067
[7]  Sun, J.Y., Guo, Q.F. and Zhuang, H.L. (1993) Two Improved MTT Methods for Assaying Vitality of Cells. Bulletin of the Academy of Military Medical Sciences, 17, 61-64.
[8]  Keum, H., Kim, T.W., Kim, Y.J., et al. (2020) Bilirubin Nanomedicine Alleviates Psoriatic Skin Inflammation by Reducing Oxidative Stress and Suppressing Pathogenic Signaling. Journal of Controlled Release, 325,359-369.
https://doi.org/10.1016/j.jconrel.2020.07.015
[9]  Harada, A., Sekido, N., Akahoshi, T., et al. (1994) Essential Involvement of Interleukin-8 (IL-8) in Acute Inflammation. Journal of Leukocyte Biology, 56, 559-564. https://doi.org/10.1002/jlb.56.5.559
[10]  Jiang F.L. and Zhang R.Q. (1989) The Research Progress on Tumour Necrosis Factor. Medical Review, 5, 306-307.
[11]  Brett, J., Gerlach, H., Nawroth, P., et al. (1989) Tumor Necrosis Factor/Cachectin Increases Permeability of Endothelial Cell Monolayers by a Mechanism Involving Regulatory G Proteins. Journal of Experimental Medicine, 169, 1977-1991.
https://doi.org/10.1084/jem.169.6.1977
[12]  Masaki, H. (2010) Role of Antioxidants in the Skin: ANTI-Aging Effects. Journal of Dermatological Science, 58, 85-90. https://doi.org/10.1016/j.jdermsci.2010.03.003
[13]  Malik, A. and Kanneganti, T.-D. (2018) Function and Regulation of IL-1α in Inflammatory Diseases and Cancer. Immunological Reviews, 281, 124-137.
https://doi.org/10.1111/imr.12615
[14]  Malleo, G., Mazzon, E., Siriwardena, A.K., et al. (2007) TNF-α as a Therapeutic Target in Acute Pancreatitis—Lessons from Experimental Models. The Scientific World Journal, 7, 431-448. https://doi.org/10.1100/tsw.2007.98

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133