%0 Journal Article
%T Optimization of LPS-Induced Inflammation Model and Its Feasibility as a Fast Screening Model for Cosmetics
%A Fanghui Sun
%A Xiaojie Song
%A Nannan Liu
%A Gang Huo
%J Journal of Cosmetics, Dermatological Sciences and Applications
%P 84-97
%@ 2161-4512
%D 2024
%I Scientific Research Publishing
%R 10.4236/jcdsa.2024.141006
%X <p>
	<b><span style=\"font-family:&quot;\">Objectives: </span></b><span style=\"font-family:&quot;\">The existing inflammatory
models are concentrated in relatively complex medical fields, and most of them
use a single type of cell, and the induction conditions are not uniform, so t</span><span style=\"font-family:&quot;\">he current LPS-induced inflammation model is less conducive to the study
of skin inflammation. The aim of this research is to enhance the existing
LPS-induced inflammation model and establish a skin inflammation model that is
suitable for the swift screening of anti-inflammatory agents in the cosmetics
industry. </span><b><span style=\"font-family:&quot;\">Methods: </span></b><span style=\"font-family:&quot;\">LPS was used to induce
inflammatory responses in KC and THP-1 cells. Enzyme-linked immunosorbent assay
(ELISA) was employed to assess the levels of IL-1<i>¦Á</i>, IL-8, and </span><span style=\"font-family:&quot;\">TNF-<i>¦Á </i></span><span style=\"font-family:&quot;\">in the two cell types, while the DCFH-DA probe was utilized to label the
levels of reactive oxygen species (ROS) in both cell types. </span><b><span style=\"font-family:&quot;\">Results:</span></b><b><span style=\"font-family:&quot;\"> </span></b><span style=\"font-family:&quot;\">In KC cells, 10 ¦Ìg/mL of LPS induced a significant upregulation of IL-8
but did not result in elevated expression of IL-1<i>¦Á</i>. However, at 100 ¦Ìg/mL of LPS, both IL-8 and IL-1<i>¦Á</i></span><i><span style=\"font-family:&quot;\"> </span></i><span style=\"font-family:&quot;\">were highly expressed in KC cells. LPS concentrations ranging from 0.01
to 100 ¦Ìg/mL failed to stimulate </span><span style=\"font-family:&quot;\">TNF-<i>¦Á </i></span><span style=\"font-family:&quot;\">production in KC cells but induced a gradient
increase in ROS levels. In THP-1 cells, LPS concentrations from 0.01 to 100
¦Ìg/mL did not induce IL-1<i>¦Á</i></span><i><span style=\"font-family:&quot;\"> </span></i><span style=\"font-family:&quot;\">production but significantly elevated IL-8 and led to a gradient increase
in </span><span style=\"font-family:&quot;\">TNF-<i>¦Á </i></span><span style=\"font-family:&quot;\">and ROS. After treatment with 100 ¦Ìg/mL of LPS, the cosmetic ingredient
Rucika KGM mitigated the elevated levels of IL-1<i>¦Á</i>, IL-8, and ROS in LPS-induced KC cells and IL-8 and ROS in THP-1
cells. </span><b><span style=\"font-family:&quot;\">Conclusion:</span></b><span style=\"font-family:&quot;\"> </span><span style=\"font-family:&quot;\">This study has successfully developed an
application-oriented
%K Cell Culture
%K Anti-Inflammatory
%K Lipopolysaccharide
%K Keratinocytes
%K THP-1
%K Inflammatory Factors
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=132078