Aim: This study aimed to investigate the protective effects
of flavonoids from the stem and leaves of Scutellaria baicalensis Georgi (SSFs)
against Aβ1-42-induced oligodendrocytes
(OL) damage. Methods: Immunofluorescence was used for the detection of myelin-associated
glycoprotein (MAG), a characteristic protein of rat oligodendrocytes (OLN-93 cells).
To evaluate the potential protective effects of SSFs on OLN-93 cells injured by
Aβ1-42, an injury model was
established by subjecting OLN-93 cells to Aβ1-42 exposed. Cell morphology was examined using an inverted microscope, while cell viability
was assessed using the colorimetric method of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide (MTT). Additionally, lactate dehydrogenase (LDH) was measured using the
pyruvic acid reduction assay. The Ginkgo biloba leaf extract (GBE) injection was
used as a positive control. Results: A total of >95% of the MAG immunofluorescence-positive
cells were identified as oligodendrocytes. Gradually increasing concentrations of
SSFs impaired the cells, and the maximum nondetrimental dose for OLN-93 cells was
75 mg/L. This study assessed the effects of SSFs on OLN-93 cells damaged by Aβ1-42. The results indicated that
SSFs significantly improved OLN-93 cell morphological abnormal changes, increased
the OLN-93 cell survival rate, and reduced LDH release. Conclusion: SSFs
can alleviate Aβ1-42-induced
damage of OL.
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