%0 Journal Article
%T The Protective Effects of Flavonoids from <i>Scutellaria Baicalensis</i> Georgi Stems and Leaves on Oligodendrocyte Damage Induced by A<i>¦Â</i><sub>1-42</sub>
%A Tangtang Song
%A Yinhui Yao
%A Yazhen Shang
%J Pharmacology & Pharmacy
%P 1-13
%@ 2157-9431
%D 2024
%I Scientific Research Publishing
%R 10.4236/pp.2024.151001
%X Aim: This study aimed to investigate the protective effects
of flavonoids from the stem and leaves of Scutellaria baicalensis Georgi (SSFs)
against A¦Â1-42-induced oligodendrocytes
(OL) damage. Methods: Immunofluorescence was used for the detection of myelin-associated
glycoprotein (MAG), a characteristic protein of rat oligodendrocytes (OLN-93 cells).
To evaluate the potential protective effects of SSFs on OLN-93 cells injured by
A¦Â1-42, an injury model was
established by subjecting OLN-93 cells to A¦Â1-42 exposed. Cell morphology was examined using an inverted microscope, while cell viability
was assessed using the colorimetric method of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide (MTT). Additionally, lactate dehydrogenase (LDH) was measured using the
pyruvic acid reduction assay. The Ginkgo biloba leaf extract (GBE) injection was
used as a positive control. Results: A total of >95% of the MAG immunofluorescence-positive
cells were identified as oligodendrocytes. Gradually increasing concentrations of
SSFs impaired the cells, and the maximum nondetrimental dose for OLN-93 cells was
75 mg/L. This study assessed the effects of SSFs on OLN-93 cells damaged by A¦Â1-42. The results indicated that
SSFs significantly improved OLN-93 cell morphological abnormal changes, increased
the OLN-93 cell survival rate, and reduced LDH release. Conclusion: SSFs
can alleviate A¦Â1-42-induced
damage of OL.
%K Flavonoids form the Stem and Leaves of <
%K i>
%K Scutellaria baicalensis<
%K /i>
%K Georgi
%K A<
%K i>
%K ¦Â<
%K /i>
%K <
%K sub>
%K 1-42<
%K /sub>
%K Oligodendrocytes
%K Damage
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=130823