Prevalence and Risk Factors of Penicillinase-Type β-Lactamase Producing Neisseria gonorrhoeae Isolated from Patients Attending Health-Facilities in Yaounde, Cameroon
Background and Objectives: Mitigation of antibiotic resistantNeisseria gonorrhoeae has become a priority due to considerable health and economical
disabilities it generates. In order to tackle the emergence of resistant Neisseria gonorrhoeae, this study aimed
to determine the prevalence and risk factors of penicillinase type β-lactamase-producing Neisseria gonorrheaeamong patients consulting for genital infectious disorders in two
health-facilities in Yaounde, Cameroon.Materials and Method: A
cross-sectional descriptive and analytical study was conducted over a 3-month
period, from July 2nd to October 2nd, 2022. Vaginal and
urethral secretions were collected. Biochemical identification tests were
performed on colonies grown on chocolate agar + polyvitex using the Api NH gallery. The detection of penicillinases was
equally performed using the API NH gallery and confirmed using the
antimicrobial susceptibility testing. The Minimum Inhibitory Concentrations of
some antibiotics were determined using the E-Test. Results: The results
showed that out of the 198 patients sampled,
16 (8.08%) were positive for Neisseria
gonorrhoeae, among which 13/16 (81.25%) were penicillinase-typeβ-lactamase producers. Antimicrobial
susceptibility testing results showed high co-resistances to antibiotics, mainly ciprofloxacin (100%),
nalidixic acid (92.31%) and azithromycin
(84.62%). Moreover, high Minimum Inhibitory Concentrations of ceftriaxone
(ranging from 6 to 24 mg/L) was observed toward Neisseria gonorrhoeae isolates. The risk factorsof
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