Albinism is a common problem encountered by researchers in anther/microspore
cultures of cereal crops. The present study investigates the effects of temperature
variations on embryogenesis of wheat (Triticum
aestivum L.) microspores. Following a cold (4°C - 13°C) vs. heat (33°C) shock to wheat tillers, microspores were
isolated and cultured in a liquid medium to obtain embryoids. Data on
embryogenic microspore%, embryoid yield, plant regeneration% and green plant%
were collected and analyzed.Cold pretreatment of
4°C or 10°C for a period of 6 or 10 days were more
effective than other cold temperature regimes in inducing microspore
embryogenesis. The heat shock of 33°C yielded the highest numbers of
embryogenic microspores and embryoids. The albino-prone genotypes produced
significantly higher green plant% following optimal cold shock, as compared to
the standard 33°C heat
shock. Results from present study suggest that cold shock may be a desirable
alternative for germplasm that produce lower green plant% using heat shock.
Lowered incubation temperature during embryoid development did not result in
higher green plant.
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