%0 Journal Article
%T Differential Effects of Cold and Heat Shock on Embryogenic Induction and Green Plant Regeneration from Wheat (<i>Triticum aestivum</i> L.) Microspores
%A Ming Y. Zheng
%A Allyson Fournier
%A Yujia Weng
%J American Journal of Plant Sciences
%P 308-322
%@ 2158-2750
%D 2023
%I Scientific Research Publishing
%R 10.4236/ajps.2023.143021
%X Albinism is a common problem encountered by researchers in anther/microspore
cultures of cereal crops. The present study investigates the effects of temperature
variations on embryogenesis of wheat (<i>Triticum
aestivum</i> L.) microspores. Following a cold (4<span style=\"font-family:;\" \"=\"\">ˇăC - 13ˇăC) vs. heat (33ˇăC) shock to wheat tillers, microspores were
isolated and cultured in a liquid medium to obtain embryoids. Data on
embryogenic microspore%, embryoid yield, plant regeneration% and green plant%
were collected and analyzed.<span style=\"color:red;\"> </span>Cold pretreatment of
4ˇăC or 10ˇăC for a period of 6 or 10 days were more
effective than other cold temperature regimes in inducing microspore
embryogenesis. The heat shock of 33ˇăC yielded the highest numbers of
embryogenic microspores and embryoids. The albino-prone genotypes produced
significantly higher green plant% following optimal cold shock, as compared to
the standard 33ˇăC heat
shock. Results from present study suggest that cold shock may be a desirable
alternative for germplasm that produce lower green plant% using heat shock.
Lowered incubation temperature during embryoid development did not result in
higher green plant.</span>
%K Albinism
%K Microspore Embryogenesis
%K Embryoid
%K Stress Treatment
%K Wheat
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=123957