Semi-Automated Enzymatic Determination of Ethanol in Beverages: Collaborative Study for RIDA？CUBE Ethanol

Easy and quick methods to quantify ethanol reliably in beverages are always important. In 2022, the Enzytec^TM Liquid Ethanol test kit was approved as AOAC Official Method^SM 2017.07 Final Action after a collaborative study was conducted with different beverages such as kombucha, juices, and beer. During set-up of this collaborative test, small sized companies asked to include the RIDA^®CUBE Ethanol/RIDA^®CUBE SCAN device since it is easy to use, suitable for a few samples only and contains the identical reagents as the Enzytec^TM Liquid system. It is applicable to quantify ethanol in diluted kombucha, fruit juices, and alcohol-free beer samples around 0.5% alcohol-by-volume within 12 min. The overall relative reproducibility standard deviation across a wide concentration range for kombucha, was calculated to be 6.29%. Analysis of juices and beer showed an overall higher variation with an estimated overall RSD(R) value by regression of 14.4%. The data obtained by this collaborative study show that the RIDA^®CUBE Ethanol in combination with the RIDA^®CUBE SCAN device is suitable to quantify ethanol from matrices representing important alcohol-free liquid food categories.