基于微囊包埋制备益生菌口含片的工艺研究
Process Study on the Preparation of Probiotic Oral Tablets Based on Microencapsulation

本项目通过分析目前比较热门的可使用益生菌种类，对其进行筛选、培养与组合，选用了干酪乳杆菌和鼠李糖乳杆菌两种目标菌种，研制出一种包含目的菌种，可以促进人们口腔健康和调节肠道菌群的益生菌口含片。该口含片既可以满足消费者品尝口含片的快感，又可以使口腔变得健康，肠道消化功能变得更好。本项目采用鼠李糖乳杆菌和干酪乳杆菌两种益生菌进行包埋和制片，并对这两种益生菌对于大肠杆菌和金黄色葡萄球菌两种致病菌的抑制作用进行探究。经过实验分析，两种益生菌对于大肠杆菌和金黄色葡萄球菌均有较好的抑制作用，且干酪乳杆菌和鼠李糖乳杆菌相互无拮抗作用，符合预期口含片的制备。为了保护益生菌在口腔和胃部中不被其中的各类酶或其他化学物质降解及实现其在小肠上释放，实验以可溶性淀粉吸附益生菌，再将其包埋入海藻酸钠和氯化钙、壳聚糖组成的外壁里，得到载运益生菌的淀粉微胶囊，在10x显微镜下观察到菌种已被包埋进微胶囊中，最后加之辅料制备成片剂。
In this project, by analyzing the current relatively popular species of usable probiotics, screening, culturing and combining them, two target species of Lactobacillus casei and Lactobacillus rhamnosus were selected, and a probiotic oral tablet containing the species of interest can promote people’s oral health and regulate the intestinal flora. This buccal tablet serves both to satisfy the pleasure of the consumer in tasting the buccal tablet and to make the mouth healthier and the gut digestive function better. Two probiotics, Lactobacillus rhamnosus and Lactobacillus casei, were used for embedding and tableting in this project, and the inhibitory effects of these two probiotics on two pathogenic bacteria of Escherichia coli and Staphylococcus aureus were also investigated. After experimental analysis, both probiotics showed better inhibitory effects on Escherichia coli and Staphylococcus aureus, and Lactobacillus casei and Lactobacillus rhamnosus had no antagonistic effect on each other, which was in accordance with the expected preparation of the buccal tablets. To protect the probiotic bacteria from degradation by various types of enzymes or other chemicals in the mouth and stomach and achieve their release on the small intestine, experiments have adsorbed the probiotic bacteria from soluble starch, which was then embedded into the outer wall consisting of sodium alginate and calcium chloride, chitosan, to obtain the probiotic loaded starch microcapsules, under the 10x microscope, it was observed that the species have been embedded into the microcapsules, and finally, the excipients were prepared as tablets.