An experiment was carried out to evaluate the
fungitoxic effect of Trichoderma
longibrachiatum (Rifai) metabolite on F. oxysporum, A. niger and A. tamarii.
The fungi were collected from the International Institute of Tropical Agriculture (IITA) and Nigerian Institute of Science
Laboratory Technology (NISLT). T. longibrachiatum was cultured on 1/4 strength Potato Dextrose Broth (PDB) following
standard procedures. Its metabolite was extracted using 50ml n-hexane with 50ml
Potato Dextrose Broth (PDB). The metabolite was purified by
filter sterilization using a sterile 0.22millipore
filter disc after centrifuging at 900rpm for 20 minutes. Petri plates of each fungus were later impregnated in triplicates
with the T. longibrachiatum metabolites using four concentrations (10%, 25%, 50% and 100%), and three
volumes (1ml, 2ml, and 3ml). Petri plates of fungi without the metabolite and Petri
Plates of fungi with n-hexane and PDB served as control. All Petri
Plates were incubated at 28°C for 7 days. Radial and diametric growth of
each fungus on all Petri Plates were taken daily at 24 hours intervals. Data obtained were
analysed using SAS
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