%0 Journal Article
%T Fungitoxicity of <i>Trichoderma longibrachiatum</i> (Rifai) Metabolites against <i>Fusarium oxysporum</i>, <i>Aspergillus niger</i> and <i>Aspergillus tamarii</i>
%A Ayodele Adegboyega Sobowale
%A Linda Chidinma Uzoma
%A Adefoyeke Olufunmilayo Aduramigba-Modupe
%A Bukola Ayodeji Bamkefa
%J American Journal of Plant Sciences
%P 984-993
%@ 2158-2750
%D 2022
%I Scientific Research Publishing
%R 10.4236/ajps.2022.137065
%X An experiment was carried out to evaluate the
fungitoxic effect of<i> Trichoderma
longibrachiatum</i> (Rifai) <i>metabolite </i>on<i> F. oxysporum</i>,<i> A. niger </i>and <i>A. tamarii</i>.
The fungi were collected from the International Institute of Tropical Agricult<span>ure (IITA) and Nigerian Institute of Science
Laboratory Technology (NISL</span><span style=\"font-family:;\" \"=\"\">T</span><span style=\"font-family:;\" \"=\"\">). <i>T. longibr</i></span><i><span style=\"font-family:;\" \"=\"\">achiatum</span></i><span style=\"font-family:;\" \"=\"\"> was cultured on </span><span style=\"font-family:;\" \"=\"\">1/4</span><span style=\"font-family:;\" \"=\"\"> strength Potato Dextrose Broth (PDB) following
standard procedures. Its metabolite was extracted using 50</span><span style=\"font-family:;\" \"=\"\"> </span><span style=\"font-family:;\" \"=\"\">ml n-hexane </span><span style=\"font-family:;\" \"=\"\">with 50</span><span style=\"font-family:;\" \"=\"\"> </span><span style=\"font-family:;\" \"=\"\">ml
Potato Dextrose Broth (PDB). The metabolite was purifi</span><span style=\"font-family:;\" \"=\"\">ed by
filter steril<span>ization using </span></span><span style=\"font-family:;\" \"=\"\">a </span><span style=\"font-family:;\" \"=\"\">sterile 0.22</span><span style=\"font-family:;\" \"=\"\"> </span><span style=\"font-family:;\" \"=\"\">millipore
filter disc after centrifuging at 900</span><span style=\"font-family:;\" \"=\"\"> </span><span style=\"font-family:;\" \"=\"\">rpm for 20 m</span><span style=\"font-family:;\" \"=\"\">inutes. Petri plates of each fungus were later impregnated in triplicates
with the <i>T. longibrachiatum</i> metabolites using four concentrations (10%, 25%, 50% and 100%), and three
volumes (1</span><span style=\"font-family:;\" \"=\"\"> </span><span style=\"font-family:;\" \"=\"\">ml, 2</span><span style=\"font-family:;\" \"=\"\"> </span><span style=\"font-family:;\" \"=\"\">ml, and 3</span><span style=\"font-family:;\" \"=\"\"> </span><span style=\"font-family:;\" \"=\"\">ml). Petr<span>i plates of fungi without the metabolite and Petri
Plates of</span> fungi with n-hexane and PDB served as control. All Petri
Plates were incubated at 28</span><span style=\"font-family:;\" \"=\"\">&deg;C for 7 days. Radial and diametric growth of
each fungus on all Petri Plates w</span><span style=\"font-family:;\" \"=\"\">ere</span><span style=\"font-family:;\" \"=\"\"> taken daily at 24 hours interval</span><span style=\"font-family:;\" \"=\"\">s</span><span style=\"font-family:;\" \"=\"\">. Data obtained were
analysed using SAS
%K Antifungal Metabolites
%K &lt
%K i&gt
%K Trichoderma longibrachiatum
%K Fusarium oxysporum
%K Aspergillus niger
%K Aspergillus tamari&lt
%K /i&gt
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=118675