Objective: To
detect the expression and significance of regulatory B cells in patients with
immune thrombocytopenia. Methods: 73 ITP patients were divided into
glucocorticoids treatment group (n = 42) and recombinant human thrombopoietin
(rhTPO) treatment group (n = 31). According to the therapeutic effect, it was
divided into effective group and ineffective group. The expression of CD19+
CD24hiCD38hi Breg in peripheral blood was detected by flow cytometry before and
after treatment. The expression levels of transforming growth factor (TGF)-β1, interleukin (IL-10) and interferon
(IFN)-γ were detected by ELISA before
and after treatment. 30 volunteers were selected as the control group. Results: The expression of CD19+ CD24hiCD38hi Breg and cytokines IL-10
and TGF-β1 in 73 ITP patients before
treatment was lower than that in the control group, while the expression of
IFN-γ was higher than that in the
control group (p < 0. 05). The expression levels of CD19+ CD24hiCD38hi Breg,
IL-10 and TGF-β1 in the effective
group were significantly higher than before treatment, while the expression of
IFN-γ was significantly lower than before treatment (p < 0. 05). The expression
of CD19+ CD24hiCD38hi Breg, IFN-γ,
IL-10 and TGF-β1 in the invalid group
had no significant change compared with before treatment.
References
[1]
Cooper, N. and Ghanima, W. (2019) Immune Thrombocytopenia. The New England Journal of Medicine, 381, 945-955. https://doi.org/10.1056/NEJMcp1810479
[2]
Lambert, M.P. and Gernsheimer, T.B. (2017) Clinical Updates in Adult Immune Thrombocytopenia. Blood, 129, 2829-2835. https://doi.org/10.1182/blood-2017-03-754119
[3]
Mohd, J.F., Garcia, S.G., Borras, F.E., Chan, G. and Franquesa, M. (2019) Paradoxical Role of Breg-Inducing Cytokines in Autoimmune Diseases. Journal of Translational Autoimmunity, 2, Article No. 100011. https://doi.org/10.1016/j.jtauto.2019.100011
[4]
Girard, M.C., Acevedo, G.R., Ossowski, M.S., et al. (2021) Ex Vivo Characterization of Breg Cells in Patients with Chronic Chagas Disease. Scientific Reports, 11, Article No. 5511. https://doi.org/10.1038/s41598-021-84765-x
[5]
Manna, A., Kellett, T., Aulakh, S., et al. (2020) Targeting CD38 is Lethal to Breg-Like Chronic Lymphocytic Leukemia Cells and Tregs, But Restores CD8+ T-Cell Responses. Blood Advances, 4, 2143-2157. https://doi.org/10.1182/bloodadvances.2019001091
[6]
Guo, L., Ma, D.X. and Hou, M. (2010) The Effects of Corticosteroid Treatment on Immune Thrombocytopenia under New Diagnostic Criteria. Chinese Journal of Internal Medicine, 49, 1020-1023.
[7]
Wang, Y., Jia, X., Zhou, L., et al. (2021) Increased let-7b-5p is Associated with Enhanced BAFF-R Expression and B Cell Survival in Immune Thrombocytopenia. International Immunopharmacology, 93, Article No. 107393.
[8]
Ray, A. and Dittel, B.N. (2017) Mechanisms of Regulatory B Cell Function in Autoimmune and Inflammatory Diseases beyond IL-10. Journal of Clinical Medicine, 6, 1-7. https://doi.org/10.1016/j.intimp.2021.107393
[9]
Stimpson, M.L., Lait, P., Schewitz-Bowers, L.P., et al. (2020) IL-10 and IL-17 Expression by CD4(+) T Cells Is Altered in Corticosteroid Refractory Immune Thrombocytopenia (ITP). Journal of Thrombosis and Haemostasis, 18, 2712-2720. https://doi.org/10.1111/jth.14970
[10]
Mokhtar, G.M., El-Beblawy, N.M., Adly, A.A., Elbarbary, N.S., Kamal, T.M. and Hasan, E.M. (2016) Cytokine Gene Polymorphism [Tumor Necrosis Factor-Alpha (−308), IL-10 (−1082), IL-6 (−174), IL-17F, 1RaVNTR] in Pediatric Patients with Primary Immune Thrombocytopenia and Response to Different Treatment Modalities. Blood Coagulation & Fibrinolysis, 27, 313-323. https://doi.org/10.1097/MBC.0000000000000431
[11]
Carvajal, A.G., Cornec, D., Saraux, A., et al. (2021) Abatacept Promotes Regulatory B Cell Functions, Enhancing Their Ability to Reduce the Th1 Response in Rheumatoid Arthritis Patients through the Production of IL-10 and TGF-Beta. Journal of Immunology, 207, 470-482. https://doi.org/10.4049/jimmunol.2000455
