%0 Journal Article
%T Expression and Significance of Regulatory B Cells in Patients with Immune Thrombocytopenia
%A Wei Qian
%A Xiaoxia Zhang
%J Open Journal of Blood Diseases
%P 45-51
%@ 2164-3199
%D 2022
%I Scientific Research Publishing
%R 10.4236/ojbd.2022.122005
%X <b><span style=\"font-family:Verdana;\">Objective:</span></b><span style=\"font-family:&quot;\"><span style=\"font-family:Verdana;\"> To
detect the expression and significance of regulatory B cells in patients with
immune thrombocytopenia. </span><b><span style=\"font-family:Verdana;\">Methods: </span></b><span style=\"font-family:Verdana;\">73 ITP patients were divided into
glucocorticoids treatment group (n = 42) and recombinant human thrombopoietin
(rhTPO) treatment group (n = 31). According to the therapeutic effect, it was
divided into effective group and ineffective group. The expression of CD19+
CD24hiCD38hi Breg in peripheral blood was detected by flow cytometry before and
after treatment. The expression levels of transforming growth factor (TGF)-</span><i><span style=\"font-family:Verdana;\">¦Â</span></i><span style=\"font-family:Verdana;\">1, interleukin (IL-10) and interferon
(IFN)-</span><i><span style=\"font-family:Verdana;\">¦Ã</span></i><span style=\"font-family:Verdana;\"> were detected by ELISA before
and after treatment. 30 volunteers were selected as the control group. </span><b><span style=\"font-family:Verdana;\">Results:</span></b><span style=\"font-family:Verdana;\"> The expression of CD19+ CD24hiCD38hi</span></span><span style=\"font-family:&quot;\"><span style=\"font-family:Verdana;\"> Breg and cytokines IL-10
and TGF-</span><i><span style=\"font-family:Verdana;\">¦Â</span></i><span style=\"font-family:Verdana;\">1 in 73 ITP patients before
treatment was lower than that in the control group, while the expression of
IFN-</span><i><span style=\"font-family:Verdana;\">¦Ã</span></i><span style=\"font-family:Verdana;\"> was higher than that in the
control group (p &lt; 0. 05). The expression levels of CD19+ CD24hiCD38hi Breg,
IL-10 and TGF-</span><i><span style=\"font-family:Verdana;\">¦Â</span></i><span style=\"font-family:Verdana;\">1 in the effective
group were significantly higher than before treatment, while the expression of
IFN-</span><i><span style=\"font-family:Verdana;\">¦Ã</span></i><span style=\"font-family:Verdana;\"> was </span><span style=\"font-family:Verdana;\">significantly lower than before treatment (p &lt; 0. 05). The expression
of CD19</span><span style=\"font-family:Verdana;\">+ CD24hiCD38hi Breg, IFN-</span><i><span style=\"font-family:Verdana;\">¦Ã</span></i><span style=\"font-family:Verdana;\">,
IL-10 and TGF-</span><i><span style=\"font-family:Verdana;\">¦Â</span></i><span style=\"font-family:Verdana;\">1 in the invalid group
had no significant change compared with before treatment. </span><b><span
%K Immune Thrombocytopenia
%K CD19+ CD24hiCD38hi Breg
%K Cytokines
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=118304