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基于高效液相色谱–串联质谱法同时测定沉积物中磺胺类抗生素及其代谢物
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Abstract:
建立了一种同时快速提取并检测沉积物中多种磺胺类抗生素及其典型代谢产物的方法，沉积物样品经冷冻干燥后，经萃取液(纯水，乙腈)萃取、净化盐(硫酸镁，C18)净化后，利用高效液相色谱–串联质谱仪(LC-MS/MS)进行测定。和常用的加速溶剂萃取法相比，该方法使用的有机溶剂量降低了57%，提取时间减少了40%。此外，该方法在低浓度下的回收率都相对较高，范围在为80%~121%，相对标准偏差小于9%。该方法可应用于水体沉积物中磺胺类抗生素及其代谢物的同时检测，且检测过程相对快速。
A rapid method was established to simultaneously extract and detect various sulfonamide antibiotics and their typical metabolites in sediments. After freeze-drying, the sediment samples were extracted with extraction solution (pure water, acetonitrile) and purified with purification salt (magnesium sulfate, C18). The target compounds were then measured by high performance liquid chromatography-tandem mass spectrometer (LC-MS/MS). Compared with the accelerated solvent extraction method, the used organic solvent in the present method was reduced by 57%, and the extraction time was reduced by 40%. In addition, the recoveries of the method at low concentrations were relatively high, ranging from 80% to 121%, and the relative standard deviations were all less than 9%. It suggests that the method could be applied to simultaneously detect sulfonamide antibiotics and their metabolites in sediments, and the detection process is relatively fast.
| [1] | Baran, W., Adamek, E., Ziemiańska, J. and Sobczak, A. (2011) Effects of the Presence of Sulfonamides in the Envi-ronment and Their Influence on Human Health. Journal of Hazardous Materials, 196, 1-15.
https://doi.org/10.1016/j.jhazmat.2011.08.082 |
| [2] | Nunes, O.C., Manaia, C.M., Kolvenbach, B.A. and Corvini, P.F.X. (2020) Living with Sulfonamides: A Diverse Range of Mechanisms Observed in Bacteria. Applied Microbiology and Biotechnology, 104, 10389-10408. |
| [3] | Vila-Costa, M., Gioia, R., Ace？a, J., Pérez, S., Casamayor, E.O. and Dachs, J. (2017) Degradation of Sulfonamides as a Microbial Resistance Mechanism. Water Research, 115, 309-317. https://doi.org/10.1016/j.watres.2017.03.007 |
| [4] | Hamid, N., Junaid, M. and Pei, D.S. (2020) Individual and Combined Mechanistic Toxicity of Sulfonamides and Their Implications for Ecological Risk Assessment in the Three Gorges Reservoir Area (TGRA), China. Journal of Hazardous Materials, 382, Article ID: 121106. https://doi.org/10.1016/j.jhazmat.2019.121106 |
| [5] | Shimizu, A., Takada, H., Koike, T., Takeshita, A., Saha, M., Nakada, N., et al. (2013) Ubiquitous Occurrence of Sulfonamides in Tropical Asian Waters. Science of the Total Envi-ronment, 452-453, 108-115.
https://doi.org/10.1016/j.scitotenv.2013.02.027 |
| [6] | Chen, Y., Zhou, J.L., Cheng, L., Zheng, Y.Y. and Xu, J. (2017) Sediment and Salinity Effects on the Bioaccumulation of Sulfamethoxazole in Zebrafish (Danio rerio). Chem-osphere, 180, 467-475.
https://doi.org/10.1016/j.chemosphere.2017.04.055 |
| [7] | Cui, J., Fu, L., Tang, B., Bin, L., Li, P., Huang, S. and Fu, F. (2020) Occurrence, Ecotoxicological Risks of Sulfonamides and Their Acetylated Metabolites in the Typical Wastewater Treatment Plants and Receiving Rivers at the Pearl River Delta. Science of the Total Environment, 709, Article ID: 136192.
https://doi.org/10.1016/j.scitotenv.2019.136192 |
| [8] | Yuan, S.F., Liu, Z.H., Yin, H., Dang, Z., Wu, P.X., Zhu, N.W. and Lin, Z. (2019) Trace Determination of Sulfonamide Antibiotics and Their Acetylated Metabolites via SPE-LC-MS/MS in Wastewater and Insights from Their Occurrence in a Municipal Wastewater Treatment Plant. Science of the Total Environment, 653, 815-821.
https://doi.org/10.1016/j.scitotenv.2018.10.417 |
| [9] | Chen, J. and Xie, S. (2018) Overview of Sulfonamide Bio-degradation and the Relevant Pathways and Microorganisms. Science of the Total Environment, 640-641, 1465-1477. https://doi.org/10.1016/j.scitotenv.2018.06.016 |
| [10] | Dmitrienko, S.G., Kochuk, E.V., Apyari, V.V., Tolmacheva, V.V. and Zolotov, Y.A. (2014) Recent Advances in Sample Preparation Techniques and Methods of Sulfonamides Detection—A Review. Analytica Chimica Acta, 850, 6-25.
https://doi.org/10.1016/j.aca.2014.08.023 |
| [11] | Ngo, T.H., Van, D.A., Tran, H.L., Nakada, N., Tanaka, H. and Huynh, T.H. (2021) Occurrence of Pharmaceutical and Personal Care Products in Cau River, Vietnam. Environmental Science and Pollution Research, 28, 12082-12091.
https://doi.org/10.1007/s11356-020-09195-0 |
| [12] | Llompart, M., Celeiro, M. and Dagnac, T. (2019) Micro-wave-Assisted Extraction of Pharmaceuticals, Personal Care Products and Industrial Contaminants in the Environment. TrAC Trends in Analytical Chemistry, 116, 136-150.
https://doi.org/10.1016/j.trac.2019.04.029 |
| [13] | 孟明辉, 贺泽英, 徐亚平, 王璐, 彭祎, 刘潇威. QuEChERS方法结合高效液相色谱-串联质谱法同时测定土壤中20种抗生素[J]. 农业环境科学学报, 2017, 36(8): 1672-1679. |
| [14] | 王大鹏, 张娴, 颜昌宙. 高效液相色谱串联质谱法测定污水污泥中4种磺胺类药物及其乙酰化代谢物[J]. 环境化学, 2018, 37(10): 2143-2151. |
