This study has established
an efficient and reproducible protocol to micropropagation Kaempferia angustifolia Roscoe via direct regeneration. The use of
young shoots as explants showed the best results compared to rhizome shoots,
where the young shoots showed a low percentage of contamination of 10% - 30%
(agar 6 g/L) and 45% - 55% (agar 3 g/L), respectively, compared to the use of
rhizome shoots, where the contamination rate exceeded 80%. For shoot
initiation, the combination of BAP (6 Benzylaminopurine) and NAA
(1-Naphthaleneacetic acid) showed higher results for the percentage of initial
shoots and number of micro shoots/explants compared to BAP with Kin (Kinetin).
The highest concentration of BAP (5 mg/L) combined with the lowest
concentration of NAA (0.5 mg/L) resulted in 90% of initial shoots and a number
of shoots/explants of 5.8. The highest number of shoots for micropropagation
was in treatment with 30 g/L sucrose that was segmented with 3 mg/L BAP + 0.5
mg/L NAA. For the number of roots, the highest
number of roots was 11.8 recorded at sucrose (45) with only BAP (1 mg/L) used
as the plant growth regulator, while the longest length of roots was 7 - 8 cm,
recorded both at sucrose with the combination of BAP and NAA.
References
[1]
Pancharoen, O., Prawat, U. and Tuntiwachwuttikul, P. (2000) Phytochemistry of the Zingiberaceae. Studies in Natural Products Chemistry, 23, 797-865.
https://doi.org/10.1016/S1572-5995(00)80142-8
[2]
Yeap, Y.S.Y., Kassim, N.K., Ng, R.C., Ee, G.C.L., Saiful Yazan, L. and Musa, K.H. (2017) Antioxidant Properties of Ginger (Kaempferia angustifolia Rosc.) and Its Chemical Markers. International Journal of Food Properties, 20, 1158-1172.
https://doi.org/10.1080/10942912.2017.1286508
[3]
Ashton, H. (2004) The Royal Horticultural Society New Encyclopedia of Herbs & Their Uses (Revised Edition) London: Dorling Kindersley 2002. 448 pp., ISBN: 1 45053 0059 0 ￡30. Reference Reviews, 18, 42-43.
https://doi.org/10.1108/09504120410528289
[4]
Tewtrakul, S. and Subhadhirasakul, S. (2007) Anti-Allergic Activity of Some Selected Plants in the Zingiberaceae Family. Journal of Ethnopharmacology, 109, 535-538.
https://doi.org/10.1016/j.jep.2006.08.010
[5]
Edwards, H. (2003) Plant Resources of Southeast Asia (PROSEA) No. 17: Fibre Plants. Phytochemistry, 64, 1174-1175.
https://doi.org/10.1016/j.phytochem.2003.08.006
[6]
Tang, S.W., Sukari, M.A., Neoh, B.K., Yeap, Y.S.Y., Abdul, A.B., Kifli, N. and Cheng Lian Ee, G. (2014) Phytochemicals from Kaempferia angustifolia Rosc. and Their Cytotoxic and Antimicrobial Activities. BioMed Research International, 2014, Article ID: 417674. https://doi.org/10.1155/2014/417674
[7]
Moreno-Vázquez, S., Larrañaga, N., Uberhuaga, E.C., Braga, E.J.B. and Pérez-Ruíz, C. (2014) Bacterial Contamination of in Vitro Plant Cultures: Confounding Effects on Somaclonal Variation and Detection of Contamination in Plant Tissues. Plant Cell, Tissue and Organ Culture (PCTOC), 119, 533-541.
https://doi.org/10.1007/s11240-014-0553-x
[8]
Nayak, S. (2000) In Vitro Multiplication and Microrhizome Induction in Curcuma aromatica Salisb. Plant Growth Regulation, 32, 41-47.
https://doi.org/10.1023/A:1006307316393
[9]
Ceylan, Y., Ozdemir, F.A. and Bulbul, A.S. (2017) Effect of Different Concentrations of BAP and NAA on Micropropagation of Crambe orientalis L. var. orientalis L. Journal of Animal and Plant Sciences, 27, 1671-1677.
[10]
Yusuf, N.A., Annuar, M.S. and Khalid, N. (2011) Rapid Micropropagation of Boesenbergia rotunda (L.) Mansf. Kulturpfl. (a Valuable Medicinal Plant) from Shoot Bud Explants. African Journal of Biotechnology, 10, 1194-1199.
[11]
Walia, N., Kaur, A. and Babbar, S.B. (2006) An Efficient, in Vitro Cyclic Production of Shoots from Adult Trees of Crataeva nurvala Buch. Ham. Plant Cell Reports, 26, 277-284. https://doi.org/10.1007/s00299-006-0239-x
[12]
Chirangini, P. and Sharma, P.J. (2005) In Vitro Propagation and Microrhizome Induction in Zingiber cassumunar (Roxb.)—An Antioxidant-Rich Medicinal Plant. Journal of Food, Agriculture & Environment, 3, 139-142.
[13]
Hill, K. and Schaller, G.E. (2013) Enhancing Plant Regeneration in Tissue Culture. Plant Signaling & Behavior, 8, e25709. https://doi.org/10.4161/psb.25709
[14]
Firoozabady, E. and Gutterson, N. (2003) Cost-Effective in Vitro Propagation Methods for Pineapple. Plant Cell Reports, 21, 844-850.
https://doi.org/10.1007/s00299-003-0577-x
[15]
Yuniastuti, E., Widodo, C.E., Samanhudi and Delfianti, M.N.I. (2018) Effect of Benzyl Amino Purine and Indole-3-acetic Acid on Propagation of Sterculia foetida in Vitro. IOP Conference Series: Earth and Environmental Science, 142, Article ID: 012011. https://doi.org/10.1088/1755-1315/142/1/012011
[16]
Lestari, K.D., Deswiniyanti, N.W., Astarini, I.A. and Arpiwi, L.M. (2019) Callus and Shoot Induction of Leaf Culture Lilium longiflorum with NAA and BAP. Nusantara Bioscience, 11, 162-165. https://doi.org/10.13057/nusbiosci/n110209
[17]
Sari, H.S., Murni, D. and Iman, B. (2015) Efek NAA dan BAP terhadap pembentukan tunas, daun, dan tinggi tunas stek mikro Nepenthes ampullaria Jack. Biosfera, 32, 194-201. (In Indonesian)
[18]
Kristina, N.N. (2009) Induction of Tabato barito (Ficus deltoidea Jack) Shoots in Vitro Using Benzyl Adenine (BA) and Naphthalene Acetic Acid (NAA). Industrial Crops Research journal, 15, 33-39.
https://doi.org/10.21082/jlittri.v15n1.2009.33-39
