%0 Journal Article
%T Micropropagation of <i>Kaempferia angustifolia</i> Roscoe via Direct Regeneration
%A Zuraida Ab Rahman
%A Ayu Nazreena Othman
%A Mohd Norfaizal Ghazalli
%A Nur Auni Syazalien Adlan
%J American Journal of Plant Sciences
%P 734-743
%@ 2158-2750
%D 2022
%I Scientific Research Publishing
%R 10.4236/ajps.2022.136049
%X This study has established
an efficient and reproducible protocol to micropropagation <i>Kaempferia angustifolia</i> Roscoe via direct regeneration. The use of
young shoots as explants showed the best results compared to rhizome shoots,
where the young shoots showed a low percentage of contamination of 10% - 30%
(agar 6 g/L) and 45% - 55% (agar 3 g/L), respectively, compared to the use of
rhizome shoots, where the contamination rate exceeded 80%. For shoot
initiation, the combination of BAP (6 Benzylaminopurine) and NAA
(1-Naphthaleneacetic acid) showed higher results for the percentage of initial
shoots and number of micro shoots/explants compared to BAP with Kin (Kinetin).
The highest concentration of BAP (5 mg/L) combined with the lowest
concentration of NAA (0.5 mg/L) resulted in 90% of initial shoots and a number
of shoots/explants of 5.8. The highest number of shoots for micropropagation
was in treatment with 30 g/L sucrose that was segmented with 3 mg/L BAP + 0.5
mg/L NAA. For the number of roots, the hig<span>hest
number of roots was 11.8 recorded at sucrose (45) with only BAP (1 mg/L) used
as the plant growth regulator, while the longest length of roots was 7 - 8 cm,
recorded both at sucrose with the combination of BAP and NAA.
%K BAP
%K NAA
%K Micropropagation
%K &lt
%K i&gt
%K Kaempferia angustifolia&lt
%K /i&gt
%K Direct Regeneration
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=117868