In this study the antioxidant activity of barley malt rootlet (BMR)
extracts was evaluated in heat treated corn oil up to 5 hours at 185°C
frying temperature. The antioxidant activity of BMR extracts was measured at 25, 50, 100 and 150 ppm
concentrations. The free and bound antioxidant phenolics were extracted from
BMR using three different extraction methods. Conventional solvent extraction
(CSE), microwave assisted extraction (MAE) and autoclave assisted pretreated
solvent extraction (APSE). In the present experiment, the total phenolic
content and antioxidant activity of the various extracts were measured. Thiobarbituric
acid reactive substances (TBARS) assay was used to evaluate the ability of the
BMR to protect lipid peroxidation in corn oil at 185°C frying temperature. The
formation of TBARS at 5 hours of heat treated corn oil has shown similar antioxidant
levels in 150 ppm butylated hydroxytoluene (BHT) or MAE free phenolic extract
added to corn oil. TBARS value for BHT was 1.896 ± 0.013 μg/mL of corn oil and
for MAE was 1.896 ± 0.034 μg/mL of corn oil. The highest level of antioxidant
activity was found for the free phenolic extracts. The order of inhibition of
oxidation was found to be for free phenolics as follows: BHT (100 ppm) >
APSE (50 ppm) > MAE (100 ppm) > CSE (100 ppm).
References
[1]
Devatkal, S.K. and Naveen, B.M. (2010) Effect of Salt, Kinnow and Pomegranate Fruit By-Product Powders on Color and Oxidative Stability of Raw Ground Goat Meat during Refrigerated Storage. Meat Science, 85, 306-311. https://doi.org/10.1016/j.meatsci.2010.01.019
[2]
Imida, K., Fukushima, S., Shivai, T., Ohtani, M., Nakamishi, K. and Ito, N. (1983) Promoting Activities of Butylated Hydroxyanisole and Butylated Hydroxytoluene on 2-Stage Urinary Bladder Carcinogenesis and Inhibition of γ-Glutamyl Tanspeptidasepositive Foci Development in the Liver of Rats. Carcinogenesis, 4, 885-889. https://doi.org/10.1093/carcin/4.7.895
[3]
Ito, N., Hirose, M., Fukushima, H., Tsuda, T., Shirai, T. and Tatenatsu, M. (1986) Studies on Antioxidants: Their Carcinogenic and Modifying Effects on Chemical Carcinogens. Food and Chemical Toxycology, 24, 1071-1092. https://doi.org/10.1016/0278-6915(86)90291-7
[4]
Witschi, H.P. (1986) Enhanced Tumour Development by Butylated Hydroxytoluene (BHT) in the Liver, Lung and Gastro-Intestinal Tract. Food and Chemical Toxicology, 24, 1127-1130. https://doi.org/10.1016/0278-6915(86)90298-X
[5]
Budaraju, S., Mallikarjunan, P.K., Annor, G., Schoenfuss, T. and Raun, R. (2018) Effect of Pretreatments on the Antioxidant Potential of Barley Malt Rootlets. Journal of Food Chemistry, 15, 31-37. https://doi.org/10.1016/j.foodchem.2018.05.110
[6]
Bai, X.L., Yue, T.L., Yuan, Y.H. and Zhang, H.W. (2010) Optimization of Microwave Assisted Extraction of Polyphenols from Apple Pomace Using Response Surface Methodology and HPLC Analysis. Journal of Separation Science, 33, 3751-3758. https://doi.org/10.1002/jssc.201000430
[7]
Sun, Y., Liao, X., Wang, Z., Hu, X. and Chen, F. (2007) Optimization of Microwave-Assisted Extraction of Anthocyanins in Red Raspberries and Identification of Anthocyanin of Extracts Using High-Performance Liquid Chromatography-Mass Spectrometry. European Food Research and Technology, 225, 511-523. https://doi.org/10.1007/s00217-006-0447-1
[8]
Pan, X., Niu, G. and Liu, H. (2003) Microwave-Assisted Extraction of Tea Polyphenols and Tea Caffeine from Green Tea Leaves. Chemical Engineering and Processing, 42, 129-133. https://doi.org/10.1016/S0255-2701(02)00037-5
[9]
Oufnac, D.S., Xu, Z., Sun, T., Sabliov, C., Prinyawiwatkul, W. and Godber, J.S. (2007) Extraction of Antioxidants from Wheat Bran Using Conventional Solvent and Microwave-Assisted Methods. Cereal Chemistry, 84, 125-129. https://doi.org/10.1094/CCHEM-84-2-0125
[10]
Zigoneanu, I.G., Williams, L., Xu, Z. and Sabliov, C.M. (2008) Determination of Antioxidant Components in Rice Bran Oil Extracted by Microwave-Assisted Method. Bioresource Technology, 99, 4910-4918. https://doi.org/10.1016/j.biortech.2007.09.067
[11]
Inglett, G.E., Rose, D.J., Stevenson, D.C., Chen, D. and Biswas, A. (2009) Total Phenolics and Antioxidant Activity of Water and Ethanolic Extracts from Distillers Dried Grains with Solubles with or without Microwave Irradiation. Cereal Chemistry, 86, 661-664. https://doi.org/10.1094/CCHEM-86-6-0661
[12]
Budaraju, S. and Mallikarjunan, P.K. (2017) Comparison and Optimization of Solvent Extraction and Microwave Assisted Extraction of Phenolic Compounds from Spent Coffee Grounds. Transactions of ASABE Paper No. 1700127. ASABE, St. Joseph. https://doi.org/10.13031/aim.201700127
[13]
Salama, A.R.A., El-Sahn, M.A., Mesallam, A.S. and Shehata, A.M.E.T. (1997) The Chemical Composition, the Nutritive Value and the Functional Properties of Malt Sprout and Its Components (Acrospires, Rootlets and Husks). Journal of Food Science and Agriculture, 75, 50-56. https://doi.org/10.1002/(SICI)1097-0010(199709)75:1<50::AID-JSFA833>3.0.CO;2-0
[14]
Bonnely, S., Peyrat-Maillard, M.N., Rondini, N., Masy, L. and Claudette, B. (2000) Antioxidant Activity of Malt Rootlet Extracts. Journal of Agricultural and Food Chemistry, 48, 2785-2792. https://doi.org/10.1021/jf990793c
[15]
Dewanto, V., Wu, X., Adom, K. and Liu, R.H. (2002) Thermal Processing Enhances the Nutritional Value of Tomatoes by Increasing Total Antioxidant Activity. Journal of Agricultural and Food Chemistry, 50, 3010-3014. https://doi.org/10.1021/jf0115589
[16]
Guo, W. and Beta, T. (2013) Phenolic Acid Composition and Antioxidant Potential of Insoluble and Soluble Dietary Fibre Extracted Derived from Select Whole Grain Cereals. Food Research International, 51, 518-525. https://doi.org/10.1016/j.foodres.2013.01.008
[17]
Fogarasi, A.L., Kun, S., Tanko, G., Stefanovits-Banya, E. and Hegysene-Vecseri, B. (2015) A Comparative Assessment of Antioxidant Properties, Total Phenolic Content of Einkorn, Wheat, Barley and Their Malts. Food Chemistry, 167, 1-6. https://doi.org/10.1016/j.foodchem.2014.06.084
[18]
Li, W., Shan, F., Sun, S., Corke, H. and Beta, T. (2005) Free Radical Scavenging Propertiesand Phenolic Content of Chinese Black-Grained Wheat. Journal of Agricultural and Food Chemistry, 53, 8533-8536. https://doi.org/10.1021/jf051634y
[19]
Duh, P.D., Yen, G.C., Yen, W.J. and Chang, L.W. (2001) Antioxidant Effects of Water Extracts from Barley (Hordeum vulgare L.) Prepared under Different Roasting Temperatures. Journal of Agriculture and Food Chemistry, 49, 1455-1463. https://doi.org/10.1021/jf000882l
[20]
Buege, J.A. and Aust, S.D. (1976) Lactoperoxidae-Catalyzed Lipid Peroxidation of Microsomal and Artificial Membranes. Biochimica et Biophysica Acta (BBA)-General Subjects, 444, 192-201. https://doi.org/10.1016/0304-4165(76)90236-1
[21]
Lee, S.C., Kim, J.H., Jeong, S.M., Kim, D.R., Ha, J.U., Nam, K.C. and Ahn, D.U. (2003) Effect of Far-Infrared Radiation on the Antioxidant Activity of Rice Hulls. Journal of Agriculture and Food Chemistry, 51, 4400-4403. https://doi.org/10.1021/jf0300285
[22]
Nicoli, M.C., Anese, M., Manzocco, L. and Lerici, C.R. (1997) Antioxidant Properties of Coffee Brews in Relation to the Roasting Degree. Lebensmittel-Wissenschaft & Technologie, 30, 292-297. https://doi.org/10.1006/fstl.1996.0181
[23]
Niwa, Y., Kanoh, T., Kasama, T. and Neigishi, M. (1988) Activation of Antioxidant Activity in Natural Medicinal Products by Heating, Brewing and Lipophilization. A New Drug Delivery System. Drugs under Experimental and Clinical Research, 14, 361-372.
