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近红外光谱结合化学计量学的感冒灵颗粒半成品中蒙花苷含量检测研究
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Abstract:
目的：本研究旨在建立近红外光谱法快速检测感冒灵颗粒半成品中蒙花苷含量的方法。方法：利用近红外光谱仪对感冒灵颗粒半成品样品进行扫描, 对其光谱进行预处理和波段选择, 并结合偏最小二乘法(partial least squares, PLS)建立浸出物含量快速无损检测方法。结果：所建立的模型的决定系数R为0.9428，交叉验证均方根差值为0.0115, 对验证集样品进行预测并统计分析, 预测值与真实值之间无显著差异(P > 0.05)。结论：所建立的模型准确度高, 适用于感冒灵颗粒半成品的蒙花苷含量的快速检测。
Objective: The aim of this study was to establish a method for the rapid determination of linarin in the semi-finished products of ganmaoling granules by near infrared spectroscopy. Methods: The semi-finished samples of ganmaoling granules were scanned by near-infrared spectroscopy (NIR). The spectra of the samples were pretreated and the bands were selected. A method for rapid non-destructive determination of extract content was established by using partial least squares method (PLS). Results: The determination coefficient R of the established model was 0.9428, and the root mean square difference of cross-validation was 0.0115. The samples of the validation set were pre-dicted and statistically analyzed, and there was no significant difference between the predicted value and the true value (P > 0.05). Conclusion: The established model has high accuracy and is suitable for the rapid determination of the content of linarin in the semi-finished products of ganmaoling granules.
| [1] | 徐启华, 贺蓉, 彭博, 等. 999感冒灵主要药效学及其作用机制研究[J]. 中国中药杂志, 2016, 41(8): 1388-1396. |
| [2] | 徐淑云, 卞如濂 , 陈修. 药理实验方法学[M]. 第3版. 北京: 人民卫生出版社, 2003: 906. |
| [3] | 吴洪福. 炎症细胞因子网络与疾病[J]. 青海医学院学报, 2003, 24(4): 267. |
| [4] | 龚非力. 医学免疫学[M]. 第3版. 北京: 科学出版社, 2009: 53. |
| [5] | 左泽平, 王志斌, 郭玉东, 等. 常用大鼠发热模型研究[J]. 中国比较医学杂志, 2012, 22(2): 52. |
| [6] | 李京宝, 韩峰, 于文功. 细菌生物膜研究技术[J]. 微生物学报, 2007, 47(3): 558. |
| [7] | 郭文胜. 清开灵注射液抗感染基础及临床研究进展[J]. 临床合理用药杂志, 2015, 8(1): 168. |
| [8] | 周汛, 李桂明. 10种中药的不同制剂对常见致病菌体外抗菌活性研究[J]. 中华中医药杂志, 2009, 24(2): 237. |
| [9] | 黄世杰. 生物膜和抗生素治疗: 新释药系统对细菌耐药的抗衡[J]. 国外医学药学分册, 2006, 33(1): 31. |
| [10] | H？iby, N. (2014) A Personal History of Research on Microbial Biofilms and Biofilm Infections. Pathogens and Disease, 70, 205-211. https://doi.org/10.3917/autr.070.0205 |
| [11] | 官妍, 王宁, 汪长中, 等. 中穿心莲内酯等中药有效成分对表皮葡萄球菌生物膜渗透性的比较[J]. 中国微生态学杂志, 2012, 24(1): 9. |
| [12] | 雷洪涛, 马淑骅, 姜秀新, 等. 连花清瘟胶囊抗金黄色葡萄球菌生物膜形成的研究[J]. 中国中医基础医学杂志, 2013, 19(10): 1133. |
| [13] | 朱小明, 杨家卿, 张昌峰, 等. 黄连解毒汤抗铜绿假单胞菌生物被膜及与阿奇霉素协同抗菌作用[J]. 中国实验方剂学杂志, 2012, 18(U): 155. |
| [14] | 枉前, 孙凤军, 夏培元. 亚抑菌浓度抗生素对细菌生物膜形成的影响[J]. 中国抗生素杂志, 2009, 34(4): 200. |
| [15] | Johnson, J.G., Murphy, C.N., et al. (2011) Type 3 Fimbriae and Biofilm Formation Are Regulated by the Transcriptional Regulators MrkHI in Klebsiella pneumoniae. Journal of Bacteriology, 193, 3453.
https://doi.org/10.1128/JB.00286-11 |
| [16] | 王改平, 倪梦梦, 裴香萍, 等. 高效液相色谱法测定清热止痛胶囊中蒙花苷的含量[J]. 山西中医学院学报, 2017, 18(3): 12-16. |
