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Botanical Research 2021
亚麻病毒诱导的基因沉默体系初探
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Abstract:
为研究烟草脆裂病毒(Tobacco rattle virus, TRV)介导的基因沉默(Virus-induced gene silencing, VIGS)技术是否适合在亚麻上应用，以PDS (Phytoene desaturase，八氢番茄红素脱氢酶)为标记基因，通过构建基因沉默载体，在亚麻品种大紫花中建立了TRV-VIGS体系。结果显示，pTRV2-PDS侵染植株真叶表现出明显的沿叶脉方向延伸的条状白化症状，且qRT-PCR分析发现与野生型及pTRV2空载体植物相比，pTRV2-PDS侵染的植株中PDS基因的表达均被有效抑制。证明，TRV介导的VIGS可以用于亚麻基因功能的验证研究。
In order to study whether tobacco rattle virus (TRV)-mediated virus induced gene silencing (VIGS) technology is suitable application for flax, a TRV-VIGS system is established by constructing a gene silencing vector with phytoene desaturase (PDS) as a marker gene. The results showed that qRT-PCR analysis showed that the expression of PDS gene in pTRV2-PDS infected plant is effectively inhibited compared with the control and empty plant, and the euphylla showed obvious albinism. The results showed that VIGS mediated by TRV could be used to verify the function of flax gene.
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