Objectives: Evaluate the effect of artificial oocyte activation (AOA) using calcium
ionophore (A23187) on the rate of fertilization and cleavage of embryos in
surgically retrieved sperm of patients with non-obstructive azoospermia
undergoing intracytoplasmic sperm injection (ICSI).Study design: This study was conducted on 60 infertile couples undergoing ICSI cycles
as a randomized controlled parallel group’s experimental study in a private IVF
center in Egypt from January 2018 to July 2019. ICSI cycles were divided into
two groups: Group A: includes 30 ICSI
patients with surgically retrieved sperms of non-obstructive azoospermia
treated with calcium ionophore (A23187). Group C/Control: includes 30 ICSI
patients with surgically retrieved sperms of non-obstructive azoospermia
non-treated with calcium ionophore (A23187). Results: There was
no statistical difference between both groups regarding the fertilization rate
(p = 0.853). There was no statistical difference between them regarding
implantation rate (p = 0.237). The percentage
of Class A embryos in the ca ionophore group was 81.7%, while it was 82.8% in
the control group. There was insignificant difference between them (p =
0.782). There was no statistical significant difference between the two groups
regarding the clinical pregnancy rate, it was
(56.7%) in the ca ionophore group while it was (53.3%) in the control
References
[1]
Shu, Y., Gebhardt, J., Watt, J., Lyon, J., Dasig, D. and Behr, B. (2007) Fertilization, Embryo Development, and Clinical Outcome of Immature Oocytes from Stimulated Intracytoplasmic Sperm Injection Cycles. Fertility and Sterility, 87, 1022-1027.
https://doi.org/10.1016/j.fertnstert.2006.08.110
[2]
Codreanu, D., Coricovac, A., Mirzan, L., Dracea, L., Marinescu, B. and Boleac, I. (2013) Natural Conception Following Total Fertilization Failure with Intracytoplasmic Sperm Injection in a Couple with Unexplained Infertility: A Case Report. Revista medico-chirurgicala a Societatii de Medici si Naturalisti din Iasi, 117, 431-438.
[3]
Mansour, R., Fahmy, I., Tawab, N.A., Kamal, A., El-Demery, Y., Aboulghar, M., et al. (2009) Electrical Activation of Oocytes after Intracytoplasmic Sperm Injection: A Controlled Randomized Study. Fertility and Sterility, 91, 133-139.
https://doi.org/10.1016/j.fertnstert.2007.08.017
[4]
Eftekhar, M., Mohammadian, F., Yousefnejad, F., Khani, P. and Aflatoonian, A. (2012) Effect of Calcium Ionophore on Unfertilized Oocytes after ICSI Cycles. Iranian Journal of Reproductive Medicine, 10, 83-86.
[5]
Kyono, K., Takisawa, T., Nakajo, Y., Doshida, M. and Toya, M. (2012) Birth and Follow-Up of Babies Born Following ICSI with Oocyte Activation Using Strontium Chloride or Calcium Lonophore A23187. Journal of Mammalian Ova Research, 29, 35-40. https://doi.org/10.1274/jmor.29.35
[6]
Sousa, M. and Tesarik, J. (1994) Ultrastructural Analysis of Fertilization Failure after Intracytoplasmic Sperm Injection. Human Reproduction, 9, 2374-2380.
https://doi.org/10.1093/oxfordjournals.humrep.a138455
[7]
Tejada, M.I., Mendoza, M.R., Corcostegui, B. and Benito, J.A. (1992) Factors Associated with Premature Chromosome Condensation (PCC) Following in Vitro Fertilization. Journal of Assisted Reproduction and Genetics, 9, 61-67.
https://doi.org/10.1007/BF01204117
[8]
Nasr-Esfahani, M.H., Razavi, S., Mardani, M., Shirazi, R. and Javanmardi, S. (2007) Effects of Failed Oocyte Activation and Sperm Protamine Deficiency on Fertilization Post-ICSI. Reproductive BioMedicine Online, 14, 422-429.
https://doi.org/10.1016/S1472-6483(10)60888-7
[9]
Windt, M.L., Coetzee, K., Kruger, T.F., Menkveld, R. and van der Merwe, J.P. (2002) Intracytoplasmic Sperm Injection with Testicular Spermatozoa in Men with Azoospermia. Journal of Assisted Reproduction and Genetics, 19, 53-59.
https://doi.org/10.1023/A:1014487412975
[10]
Palermo, G.D., Schlegel, P.N., Hariprashad, J.J., Ergun, B., Mielnik, A., Zaninovic, N., et al. (1999) Fertilization and Pregnancy Outcome with Intracytoplasmic Sperm Injection for Azoospermic Men. Human Reproduction, 14, 741-748.
https://doi.org/10.1093/humrep/14.3.741
[11]
Yalcin, I., Berker, B., Sukur, Y.E., Kahraman, K. and Ates, C. (2017) Comparison of Intracytoplasmic Sperm Injection with Testicular Spermatozoa Success in Infertile Men with Obstructive and Non-Obstructive Azoospermia: A Retrospective Analysis. Human Fertility (Cambridge, England), 20, 186-191.
https://doi.org/10.1080/14647273.2016.1264632
[12]
Pasqualotto, F.F., Rossi-Ferragut, L.M., Rocha, C.C., Iaconelli, A. and Borges, E. (2002) Outcome of in Vitro Fertilization and Intracytoplasmic Injection of Epididymal and Testicular Sperm Obtained from Patients with Obstructive and Nonobstructive Azoospermia. The Journal of Urology, 167, 1753-1756.
https://doi.org/10.1016/S0022-5347(05)65193-2
[13]
Vanden Meerschaut, F., Leybaert, L., Nikiforaki, D., Qian, C., Heindryckx, B. and De Sutter, P. (2013) Diagnostic and Prognostic Value of Calcium Oscillatory Pattern Analysis for Patients with ICSI Fertilization Failure. Human Reproduction, 28, 87-98. https://doi.org/10.1093/humrep/des368
[14]
Machaty, Z. (2016) Signal Transduction in Mammalian Oocytes during Fertilization. Cell and Tissue Research, 363, 169-183.
https://doi.org/10.1007/s00441-015-2291-8
[15]
Wakai, T., Vanderheyden, V. and Fissore, R.A. (2011) Ca2+ Signaling during Mammalian Fertilization: Requirements, Players, and Adaptations. Cold Spring Harbor Perspectives in Biology, 3, a006767.
[16]
Stricker, S.A. (1999) Comparative Biology of Calcium Signaling during Fertilization and Egg Activation in Animals. Developmental Biology, 211, 157-176.
https://doi.org/10.1006/dbio.1999.9340
[17]
Hafez, E.S., Goff, L. and Hafez, B. (2004) Mammalian Fertilization, IVF, ICSI: Physiological/Molecular Parameters, Clinical Application. Archives of Andrology, 50, 69-88. https://doi.org/10.1080/aan.50.2.69.88
[18]
Rice, A., Parrington, J., Jones, K.T. and Swann, K. (2000) Mammalian Sperm Contain a Ca2+-Sensitive Phospholipase C Activity That Can Generate InsP3 from PIP2 Associated with Intracellular Organelles. Developmental Biology, 228, 125-135.
https://doi.org/10.1006/dbio.2000.9929
[19]
Saunders, C.M., Swann, K. and Lai, F.A. (2007) PLCzeta, a Sperm-Specific PLC and Its Potential Role in Fertilization. Biochemical Society Symposia, 74, 23-36.
[20]
Heytens, E., Schmitt-John, T., Moser, J.M., Jensen, N.M., Soleimani, R., Young, C., et al. (2010) Reduced Fertilization after ICSI and Abnormal Phospholipase C Zeta Presence in Spermatozoa from the Wobbler Mouse. Reproductive BioMedicine Online, 21, 742-749. https://doi.org/10.1016/j.rbmo.2010.07.006
[21]
Heytens, E., Soleimani, R., Lierman, S., De Meester, S., Gerris, J., Dhont, M., et al. (2008) Effect of Ionomycin on Oocyte Activation and Embryo Development in Mouse. Reproductive BioMedicine Online, 17, 764-771.
https://doi.org/10.1016/S1472-6483(10)60403-8
[22]
Tesarik, J., Sousa, M. and Mendoza, C. (1995) Sperm-Induced Calcium Oscillations of Human Oocytes Show Distinct Features in Oocyte Center and Periphery. Molecular Reproduction and Development, 41, 257-263.
https://doi.org/10.1002/mrd.1080410217
[23]
Montag, M., Koster, M., van der Ven, K., Bohlen, U. and van der Ven, H. (2012) The Benefit of Artificial Oocyte Activation Is Dependent on the Fertilization Rate in a Previous Treatment Cycle. Reproductive BioMedicine Online, 24, 521-526.
https://doi.org/10.1016/j.rbmo.2012.02.002
[24]
Rout, U.K., Krawetz, S.A. and Armant, D.R. (1997) Ethanol-Induced Intracellular Calcium Mobilization Rapidly Alters Gene Expression in the Mouse Blastocyst. Cell Calcium, 22, 463-474. https://doi.org/10.1016/S0143-4160(97)90074-9
[25]
Ozil, J.P., Banrezes, B., Toth, S., Pan, H. and Schultz, R.M. (2006) Ca2+ Oscillatory Pattern in Fertilized Mouse Eggs Affects Gene Expression and Development to Term. Developmental Biology, 300, 534-544.
https://doi.org/10.1016/j.ydbio.2006.08.041
[26]
Dale, B., Fiorentino, A., De Stefano, R., Di Matteo, L., De Simone, M.L., Wilding, M., et al. (1999) Pregnancies after Activated Oocyte Transfer: A New Option for Infertility Treatment. Human Reproduction, 14, 1771-1772.
https://doi.org/10.1093/humrep/14.7.1771
[27]
Kim, S.T., Cha, Y.B., Park, J.M. and Gye, M.C. (2001) Successful Pregnancy and Delivery from Frozen-Thawed Embryos after Intracytoplasmic Sperm Injection Using Round-Headed Spermatozoa and Assisted Oocyte Activation in a Globozoospermic Patient with Mosaic Down Syndrome. Fertility and Sterility, 75, 445-447.
https://doi.org/10.1016/S0015-0282(00)01698-8
[28]
Eldar-Geva, T., Brooks, B., Margalioth, E.J., Zylber-Haran, E., Gal, M. and Silber, S.J. (2003) Successful Pregnancy and Delivery after Calcium Ionophore Oocyte Activation in a Normozoospermic Patient with Previous Repeated Failed Fertilization after Intracytoplasmic Sperm Injection. Fertility and Sterility, 79, 1656-1658.
[29]
Murase, Y., Araki, Y., Mizuno, S., Kawaguchi, C., Naito, M., Yoshizawa, M., et al. (2004) Pregnancy Following Chemical Activation of Oocytes in a Couple with Repeated Failure of Fertilization Using ICSI: Case Report. Human Reproduction, 19, 1604-1607. https://doi.org/10.1093/humrep/deh294
[30]
Heindryckx, B., Van der Elst, J., De Sutter, P. and Dhont, M. (2005) Treatment Option for Sperm- or Oocyte-Related Fertilization Failure: Assisted Oocyte Activation Following Diagnostic Heterologous ICSI. Human Reproduction, 20, 2237-2241.
https://doi.org/10.1093/humrep/dei029
[31]
Abramov, A.Y. and Duchen, M.R. (2003) Actions of Ionomycin, 4-BrA23187 and a Novel Electrogenic Ca2+ Ionophore on Mitochondria in Intact Cells. Cell Calcium, 33, 101-112. https://doi.org/10.1016/S0143-4160(02)00203-8
[32]
Kotz, S., Balakrishnan, N., Read, B.C. and Vidakovic, B. (2006) Encyclopedia of Statistical Sciences. Wiley-Interscience, Hoboken.
[33]
A, K.L. and Feeney, B.C. (2013) A Simple Guide to IBM SPSS Statistics for Version 20.0. Wadsworth, Cengage Learning, Belmont.
[34]
Taylor, S.L., Yoon, S.Y., Morshedi, M.S., Lacey, D.R., Jellerette, T., Fissore, R.A., et al. (2010) Complete Globozoospermia Associated with PLCzeta Deficiency Treated with Calcium Ionophore and ICSI Results in Pregnancy. Reproductive BioMedicine Online, 20, 559-564. https://doi.org/10.1016/j.rbmo.2009.12.024
[35]
Tejera, A., Molla, M., Muriel, L., Remohi, J., Pellicer, A. and De Pablo, J.L. (2008) Successful Pregnancy and Childbirth after Intracytoplasmic Sperm Injection with Calcium Ionophore Oocyte Activation in a Globozoospermic Patient. Fertility and Sterility, 90, 1202.e1-5.
[36]
Kyono, K., Nakajo, Y., Nishinaka, C., Hattori, H., Kyoya, T., Ishikawa, T., et al. (2009) A Birth from the Transfer of a Single Vitrified-Warmed Blastocyst Using Intracytoplasmic Sperm Injection with Calcium Ionophore Oocyte Activation in a Globozoospermic Patient. Fertility and Sterility, 91, 931.e7-11.
[37]
Nasr-Esfahani, M.H., Razavi, S., Javdan, Z. and Tavalaee, M. (2008) Artificial Oocyte Activation in Severe Teratozoospermia Undergoing Intracytoplasmic Sperm Injection. Fertility and Sterility, 90, 2231-2237.
https://doi.org/10.1016/j.fertnstert.2007.10.047
[38]
Moaz, M.N., Khattab, S., Foutouh, I.A. and Mohsen, E.A. (2006) Chemical Activation of Oocytes in Different Types of Sperm Abnormalities in Cases of Low or Failed Fertilization after ICSI: A Prospective Pilot Study. Reproductive BioMedicine Online, 13, 791-794. https://doi.org/10.1016/S1472-6483(10)61025-5
[39]
Borges, E., de Almeida Ferreira Braga, D.P., de Sousa Bonetti, T.C., Iaconelli, A. and Franco, J.G. (2009) Artificial Oocyte Activation Using Calcium Ionophore in ICSI Cycles with Spermatozoa from Different Sources. Reproductive BioMedicine Online, 18, 45-52. https://doi.org/10.1016/S1472-6483(10)60423-3
[40]
Stecher, A., Bach, M., Neyer, A., Vanderzwalmen, P., Zintz, M. and Zech, N.H. (2011) Case Report: Live Birth Following ICSI with Non-Vital Frozen-Thawed Testicular Sperm and Oocyte Activation with Calcium Ionophore. Journal of Assisted Reproduction and Genetics, 28, 411-414.
https://doi.org/10.1007/s10815-011-9546-4
[41]
Ebner, T., Koster, M., Shebl, O., Moser, M., Van der Ven, H., Tews, G., et al. (2012) Application of a Ready-to-Use Calcium Ionophore Increases Rates of Fertilization and Pregnancy in Severe Male Factor Infertility. Fertility and Sterility, 98, 1432-1437.
https://doi.org/10.1016/j.fertnstert.2012.07.1134
[42]
Palermo, G., Joris, H., Devroey, P. and Van Steirteghem, A.C. (1992) Pregnancies after Intracytoplasmic Injection of Single Spermatozoon into an Oocyte. The Lancet, 340, 17-18. https://doi.org/10.1016/0140-6736(92)92425-F
[43]
Craft, I., Bennett, V. and Nicholson, N. (1993) Fertilising Ability of Testicular Spermatozoa. The Lancet, 342, 864. https://doi.org/10.1016/0140-6736(93)92722-6
[44]
Heytens, E., Parrington, J., Coward, K., Young, C., Lambrecht, S., Yoon, S.Y., et al. (2009) Reduced Amounts and Abnormal Forms of Phospholipase C Zeta (PLCzeta) in Spermatozoa from Infertile Men. Human Reproduction, 24, 2417-2428.
https://doi.org/10.1093/humrep/dep207
[45]
Yoon, H.J., Bae, I.H., Kim, H.J., Jang, J.M., Hur, Y.S., Kim, H.K., et al. (2013) Analysis of Clinical Outcomes with Respect to Spermatozoan Origin after Artificial Oocyte Activation with a Calcium Ionophore. Journal of Assisted Reproduction and Genetics, 30, 1569-1575. https://doi.org/10.1007/s10815-013-0110-2
[46]
Kang, H.J., Lee, S.H., Park, Y.S., Lim, C.K., Ko, D.S., Yang, K.M., et al. (2015) Artificial Oocyte Activation in Intracytoplasmic Sperm Injection Cycles Using Testicular Sperm in Human in Vitro Fertilization. Clinical and Experimental Reproductive Medicine, 42, 45-50. https://doi.org/10.5653/cerm.2015.42.2.45
[47]
Swann, K., Larman, M.G., Saunders, C.M. and Lai, F.A. (2004) The Cytosolic Sperm Factor That Triggers Ca2+ Oscillations and Egg Activation in Mammals Is a Novel Phospholipase C: PLCzeta. Reproduction, 127, 431-439.
https://doi.org/10.1530/rep.1.00169
[48]
Dozortsev, D., Qian, C., Ermilov, A., Rybouchkin, A., De Sutter, P. and Dhont, M. (1997) Sperm-Associated Oocyte-Activating Factor Is Released from the Spermatozoon within 30 Minutes after Injection as a Result of the Sperm-Oocyte Interaction. Human Reproduction, 12, 2792-2796. https://doi.org/10.1093/humrep/12.12.2792
[49]
Bos-Mikich, A., Whittingham, D.G. and Jones, K.T. (1997) Meiotic and Mitotic Ca2+ Oscillations Affect Cell Composition in Resulting Blastocysts. Developmental Biology, 182, 172-179. https://doi.org/10.1006/dbio.1996.8468
[50]
Ozil, J.P. and Huneau, D. (2001) Activation of Rabbit Oocytes: The Impact of the Ca2+ Signal Regime on Development. Development, 128, 917-928.
https://doi.org/10.1242/dev.128.6.917
[51]
Ebner, T., Oppelt, P., Wober, M., Staples, P., Mayer, R.B., Sonnleitner, U., et al. (2015) Treatment with Ca2+ Ionophore Improves Embryo Development and Outcome in Cases with Previous Developmental Problems: A Prospective Multicenter Study. Human Reproduction, 30, 97-102. https://doi.org/10.1093/humrep/deu285
