Determination of Genetic Polymorphisms of Leptin, Ghreline and Insulin Like Growth Factor-1 (IGF-1) Genes in Beef Cattle Raised in Turkey

This study was performed to determine polymorphisms in leptin, ghrelin and insulin like growth factor-1 (IGF-1) genes and to identify genotype and alleles frequencies in beef cattles raised in Sanliurfa province of Turkey. A total of 405 male beef cattles, which were raised in Harranova Live stock and Agricultural Enterprise in ？anl？urfa and selected by phenotypic evaluation, were included in to the study. The animal material consisted of Hereford (n=112), Angus (n=145), Charolais (n=54), Black Hereford (n=36), Brahman (n=24) and Limousin (n=34) cattles. After DNA isolation from meat samples, leptin, ghrelin and insulin like growth factor-1 (IGF-1) gene polymorphisms were determined by using PCR-RFLP method. All three possible genotypes of BB, AB and AA were observed in IGF-1/SnaBI gene AB and AA genotypes had the highest and the lowest frequencies, respectively. The frequency of B allele (0.600) was higher than that of A allele (0.400). With respect to PstI polymorphism at the leptin gene, three possible genotypes were also observed. In this locus; genotypes having the highest and the lowest frequency were CT and TT respectively and the frequency of C allele was 0.571 while frequency of T was 0.429. At the BfaI polymorphic site of Ghrelin gene in AA and AG genotypes were observed while GG genotype was not observed. Accordingly, the frequency of the A allele was found to be high (0.938) and the frequency of the G allele was found to be quite low (0.062). As a result, it was determined that the examined beef cattle populations were in the Hardy-Weinberg equilibrium in terms for IGF-1 / SnaBI and Ghrelin / BfaI polymorphisms but not for Leptin/PstI polymorphism