%0 Journal Article
%T Determination of Genetic Polymorphisms of Leptin, Ghreline and Insulin Like Growth Factor-1 (IGF-1) Genes in Beef Cattle Raised in Turkey
%A Ayd£¿n DA£¿
%J -
%D 2019
%X This study was performed to determine polymorphisms in leptin, ghrelin and insulin like growth factor-1 (IGF-1) genes and to identify genotype and alleles frequencies in beef cattles raised in Sanliurfa province of Turkey. A total of 405 male beef cattles, which were raised in Harranova Live stock and Agricultural Enterprise in £¿anl£¿urfa and selected by phenotypic evaluation, were included in to the study. The animal material consisted of Hereford (n=112), Angus (n=145), Charolais (n=54), Black Hereford (n=36), Brahman (n=24) and Limousin (n=34) cattles. After DNA isolation from meat samples, leptin, ghrelin and insulin like growth factor-1 (IGF-1) gene polymorphisms were determined by using PCR-RFLP method. All three possible genotypes of BB, AB and AA were observed in IGF-1/SnaBI gene AB and AA genotypes had the highest and the lowest frequencies, respectively. The frequency of B allele (0.600) was higher than that of A allele (0.400). With respect to PstI polymorphism at the leptin gene, three possible genotypes were also observed. In this locus; genotypes having the highest and the lowest frequency were CT and TT respectively and the frequency of C allele was 0.571 while frequency of T was 0.429. At the BfaI polymorphic site of Ghrelin gene in AA and AG genotypes were observed while GG genotype was not observed. Accordingly, the frequency of the A allele was found to be high (0.938) and the frequency of the G allele was found to be quite low (0.062). As a result, it was determined that the examined beef cattle populations were in the Hardy-Weinberg equilibrium in terms for IGF-1 / SnaBI and Ghrelin / BfaI polymorphisms but not for Leptin/PstI polymorphism
%K Genetik polimorfizm
%K Besi s£¿£¿£¿rlar£¿
%K Leptin
%K Ghrelin
%K IGF-1
%U http://dergipark.org.tr/huvfd/issue/47022/592643