Bifidogenic effect of salep powder

The aim of this research was to study the bifidogenic effect of salep powder on some Bifidobacterium species, namely B. lactis, B. infantis, B. longum and B. bifidum. The growth of the Bifidobacterium species was investigated by measuring the pH and optical density (OD) during 48 h-fermentation. In order to evaluate the efficiency of the fermentation of salep powder by the Bifidobacterium subsp. lactic, acetic, butyric and propionic acid values in the growth media were quantified by HPLC. It was determined that Bifidobacterium species might be used salep powder as a source of carbon and the highest OD values were found for B. lactis in media containing glucose and for B. infantis in media containing salep powder. All the tested strains observed the ability to produce higher amounts of lactic and acetic acids than amounts of propionic and butyric acids. Consequently, it was determined that all four Bifidobacterium species were able to ferment salep powder. Moreover, because salep powder enhanced the growth and activity of Bifidobacteria and it might be conferred as a bifidogenic effect