Determination of β-Sitosterol in Alchemilla caucasica Buser by GC-MS Method

In this study, it was aimed to develop a method for the determination of β-sitosterol by gas chromatography-mass spectrometry method in Alchemilla caucasica Buser. For β-sitosterol, selected ion monitoring and electron ionization modes were used. Calibration linearity of the devoloped method was obtained at 1-100 μg/mL concentration for β-sitosterol. The intra-day and inter-day precision value for β-sitosterol was less than 4.99%. The accuracy of the method was observed to be better than 4.60%. The detection and quantification limits for β-sitosterol were found 0.05 and 0.15 μg/mL, respectively. The amount of β-sitosterol in A. caucasica plant was found to be 22.6 μg/1.0 mg with percent recovery 2.26% for β-sitosterol. Also, this method can be used for the determination of β-sitosterol in A. caucasica plant