%0 Journal Article
%T Determination of ¦Â-Sitosterol in Alchemilla caucasica Buser by GC-MS Method
%A Esen SEZEN KARAO£¿LAN
%J -
%D 2018
%X In this study, it was aimed to develop a method for the determination of ¦Â-sitosterol by gas chromatography-mass spectrometry method in Alchemilla caucasica Buser. For ¦Â-sitosterol, selected ion monitoring and electron ionization modes were used. Calibration linearity of the devoloped method was obtained at 1-100 ¦Ìg/mL concentration for ¦Â-sitosterol. The intra-day and inter-day precision value for ¦Â-sitosterol was less than 4.99%. The accuracy of the method was observed to be better than 4.60%. The detection and quantification limits for ¦Â-sitosterol were found 0.05 and 0.15 ¦Ìg/mL, respectively. The amount of ¦Â-sitosterol in A. caucasica plant was found to be 22.6 ¦Ìg/1.0 mg with percent recovery 2.26% for ¦Â-sitosterol. Also, this method can be used for the determination of ¦Â-sitosterol in A. caucasica plant
%K Alchemilla caucasica
%K ¦Â-sitosterol
%K GC-MS
%K Validation
%U http://dergipark.org.tr/erzifbed/issue/38782/412214