Reversed phase high performance liquid chromatography method for determination of Thiocolchicoside from active pharmaceutical dosage form

A simple, rapid and accurate high performance liquid chromatography method is described for determination of thiocolchicoside from active pharmaceutical ingredients. The separation of drug was achieved on Zorbax Eclipse C18 (250 x 4.6 mm i.d.) with 5 μ particle size column showed most favorable chromatographic pattern over the other columns. The mobile phase consisted of a mixture of buffer and acetonitrile (70:30 % v/v). The buffer was mixtures of 0.1 % tri-ethyl amine adjusted the pH 3.3 with ortho-phosphoric acid. The detection was carried out at wavelength 260 nm. The mixture of buffer and acetonitrile (70:30% v/v) was used as a diluent. The method was validated for system suitability, linearity, accuracy, precision, robustness, stability of sample solution. The method has been successfully used to analyze thiocolchicoside hydrochloride from active pharmaceutical ingredient