Erythropoietin/aloe vera

This study aimed to develop a recombinant human erythropoietin/aloe vera gel–releasing polyvinyl alcohol/chitosan wound dressing by wet-electrospinning method and investigate the synergistic effects of erythropoietin and aloe vera gel on the excisional wound healing. The polyvinyl alcohol/chitosan (4:1 (w/w)) solution was electrospun into an aloe vera gel–containing water coagulation bath and then coated with the recombinant human erythropoietin–loaded chitosan nanoparticles prepared via ionic gelation technique. The developed sponge-like film was able to release the erythropoietin and aloe vera gel for at least 7 and 1？days, respectively. The dressing was non-toxic to L929 fibroblastic cell line and non-adherent to the wound bed with the contact angle of 67.93°？±？2.31°. It also possessed the water-uptake capacity and water vapor transmission rate of 26.40%？±？3.37% and 2584.00？±？144.67？g？m？2, respectively. The in vivo study demonstrated the synergistic wound-healing effects of aloe vera gel and erythropoietin on the full-thickness excisional wounds of Wistar rats. The erythropoietin/aloe vera gel–releasing dressing had significantly (n？=？4, p？<？0.005) higher wound closure (92.96%？±？10.09%) than the sterile gauze (73.89%？±？2.61%), as the control group, after 2？weeks. The differences observed between the wound closure percentages of the aloe vera gel–releasing dressing (85.93%？±？6.46%) and the erythropoietin/aloe vera gel–releasing dressing and control group were not statistically significant