Ageing influences the relationship of circulating miR

The identification of circulating microRNAs related to abnormal metabolic function may be useful in the context of ageing, adiposity and insulin resistance. The miR-33a/b has been shown to control the expression of genes involved in fatty acid biosynthesis, impaired metabolism and insulin resistance. In this study, we aimed to identify differences in circulating miR-33a/b levels according to age-related metabolic impairment and increased adiposity. This study included 80 individuals (30.2% with obesity, 70% females) classified according to insulin resistance (Stern’s criteria) and age [young (20–39？years) and senior (40–59？years)]. Body fat was evaluated using bioelectrical impedance, biochemical markers by colorimetric, enzymatic and immuno-turbidimetry methods. TaqMan measures of circulating miR-33a and miR-33b with quantitative reverse transcription polymerase chain reaction in serum were assessed in association with clinical outputs. Circulating miR-33a and miR-33b levels showed significant association with fatness, the lipid profile and biomarkers of impaired glucose metabolism. Both miR-33a and miR-33b were associated with visceral adiposity index in non-insulin resistance and insulin resistance individuals. More important, for miR-33a circulating levels in senior group, receiver operating characteristic curve analyses showed area under the curve 0.804 (p？=？0.010; 95% confidence interval？=？0.655–0.952). Ageing influenced the relationship of circulating miR-33a and miR-33b with insulin resistance and increased adiposity