食管癌放射抵抗相关基因的筛选
DOI: 10.3971/j.issn.1000-8578.2019.18.1007
Keywords: 食管癌,放射敏感,放射抵抗,基因芯片,Effects of UBE2N Knockdown on Radiosensitivity of Human Laryngeal Squamous Cell Carcinoma hep-2 Cells,Effect of Selective Increase of Single Dose of PTV on Prognosis of Patients with Esophageal Cancer,Silence of STAT3 Enhances SNX-2112-induced Apoptosis of Esophageal Cancer Stem Cells,Effect of Overexpression of Follistatin-like Protein 1 on Downstream Genes in Cervical Cancer and Bioinformatic Analysis of Downstream Genes,Application of COMPASS 3D Dose Validation System in Volumetric Modulated Arc Therapy of Esophageal Cancer,Research Progress of Long Non-coding RNA in Esophageal Cancer,Apoptotic Effects of Cryptotanshinone on Human Esophageal Carcinoma HCE-4 Cells and Related Mechanism,Identification of Differently Expressed Genes and Pathways in Glioblastoma Multiforme Using Microarray,Effects of Aloin on Proliferation, Apoptosis and Invasion of Esophageal Cancer KESY70 Cell Line,Berberine Inhibits Proliferation and Induces Apoptosis of Esophageal Cancer Cells via Inactivation of IGF-1R Signaling Pathway,Prognosis of Stage ⅡB-Ⅲ Thoracic Esophageal Carcinoma Patients Treated with Prophylactic After Radical Esophagectomy,Clinical Characteristics and Prognostic Factors of 35 Patients with Esophagus Adenoid Cystic Carcinoma Patients,Impact of SLC39A5 Knockout on Establishment of Esophageal Cancer Model Induced by 4-NQO in C57BL/6 Mice,Preoperative Platelet-to-lymphocyte Ratio and Neutrophil-to-lymphocyte Ratio for Predicting Prognosis of Esophageal Cancer Patients,Clinical Comparison of Minimally Invasive Esophagectomy Versus Open Esophagectomy on Elderly Esophageal Carcinoma Patients (≥70 Years Old)
Abstract:
摘要 目的 筛选与食管癌放射抵抗相关的基因，为指导临床个体化治疗打下基础。方法 采用基 因芯片技术检测放疗疗效差异最大的两组患者之间差异明显的CNV片段，筛选出这些CNV片段中的代表性基因。进一步从筛选出的高度相关的代表性基因中，利用qPCR进行验证。结果 基因芯片筛查的结果得到显著差异的CNV片段共5个，分别位于3、4、8、11、17号染色体，其中拷贝数缺失的（CN Loss）3个，增加的（CN Gain）1个，杂合性缺失的（LOH）1个。代表性基因分别为ROBO1、NSD2、CSMD3、CADM1、NF1。qPCR验证的放射抵抗相关基因为NSD2和CADM1，主要涉及DNA损伤修复通路和细胞黏附。结论 基因芯片筛选食管癌放射抵抗相关分子标记具有可行性。NSD2和CADM1可能是与食管癌放射抵抗的相关位点
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