过表达miR-7对结肠癌HCT-116细胞生物学行为的影响
DOI: 10.3971/j.issn.1000-8578.2019.18.0971
Keywords: miR-7,结肠癌,细胞增殖,凋亡,PI3K/AKT信号通路,Autophagy Related Protein 5 Regulates Colon Cancer Cell Senescence Through p53/p21 Pathway,Advances in Role of Cyclooxygenase 2 in Development and Progression of Cancer,Effects of miR-144 on Proliferation, Migration, Invasion and PI3K Pathway of Pancreatic Cancer SW1990 Cells,Effect of ANLN Gene Silencing on Invasion, Migration, Proliferation and Apoptosis of Gastric Cancer Cells,Antitumor and Apoptosis Induction Effects of Gold Nanorods-mediated Near-infrared Photothermal Therapy on Gastric Cancer SGC-7901 Cells in vitro,Effects of MicroRNA-4465 on Apoptosis and Invasion of Colon Cancer Cells,Effect of Noscapine on Drug Resistance of 5-Fluorouracil-resistant Human Colon Cancer Cell Lines HT-29/5-Fu, LoVo/5-Fu and SW480/5-Fu,Regulatory Effect of Astragaloside A on Proliferation and Apoptosis of Human Colon Cancer SW480 Cell Line,Effects of Pachymaran on Proliferation, Migration and Pro-apoptosis of Human Cervical Carcinoma HeLa Cells and Its Mechanism,Anti-tumor Effects of Chlorogenic Acid on Human Glioblastoma Cell Lines U251 and Related Pro-apoptotic Mechanism,Downregulation of Skp2 Expression Inhibits Malignant Phenotype of Osteosarcoma U2OS Cells,Apatinib Suppresses Proliferation and Induced Apoptosis of Human Breast Cancer Cell Line MDA-MB-231 Through Glycolytic Inhibition,Effects of Long Non-coding RNA-MALAT1 on Proliferation and Apoptosis of Prostate Cancer Cells,Expression and Biological Functions of Sestrin2 in Nasopharyngeal Carcinoma,RNA PolymeraseⅠInhibitor Regulates Proliferation, Invasion and Apoptosis of Nasopharyngeal Carcinoma Cell Line CNE-1 by NF-κb
Abstract:
摘要 目的 探讨上调microRNA-7（miR-7）表达对结肠癌HCT-116细胞生物学行为的影响及可能机制。方法 HCT-116转染后分成miR-7 mimics组、Scramble（阴性对照）组和空白对照组。实时荧光定量PCR（RT-Fq-PCR）检测转染48 h后各组细胞中miR-7表达水平，CCK-8法检测转染72 h后各组细胞的增殖抑制情况，Transwell实验观察转染24 h后细胞侵袭能力，流式细胞仪行细胞周期和细胞凋亡检测。Western blot检测PI3K（p110）、p-Akt及p-mTOR的蛋白表达。结果 miR-7 mimics组miR-7表达水平明显上调，与其余两组比较差异有统计学意义（P<0.05）；与阴性对照组和空白对照组相比，miR-7 mimics组细胞增殖抑制率升高，侵袭能力降低，细胞周期分析提示G0/G1期的细胞比例增高，S期细胞明显减少，细胞凋亡率明显增加， 且PI 3K、p-Akt及p-mTOR的蛋白水平明显降低（P<0.05）。结论 上调结肠癌HCT-116细胞中miR-7表达能抑制肿瘤细胞的增殖与侵袭，其机制可能与抑制PI3K/AKT信号通路有关
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