%0 Journal Article
%T 过表达miR-7对结肠癌HCT-116细胞生物学行为的影响
%A 熊兵红
%A 王昆华
%A 罗华友
%A 马利
%A 黄卫
%J 肿瘤防治研究
%D 2019
%R 10.3971/j.issn.1000-8578.2019.18.0971
%X 摘要 目的 探讨上调microRNA-7（miR-7）表达对结肠癌HCT-116细胞生物学行为的影响及可能机制。方法 HCT-116转染后分成miR-7 mimics组、Scramble（阴性对照）组和空白对照组。实时荧光定量PCR（RT-Fq-PCR）检测转染48 h后各组细胞中miR-7表达水平，CCK-8法检测转染72 h后各组细胞的增殖抑制情况，Transwell实验观察转染24 h后细胞侵袭能力，流式细胞仪行细胞周期和细胞凋亡检测。Western blot检测PI3K（p110）、p-Akt及p-mTOR的蛋白表达。结果 miR-7 mimics组miR-7表达水平明显上调，与其余两组比较差异有统计学意义（P<0.05）；与阴性对照组和空白对照组相比，miR-7 mimics组细胞增殖抑制率升高，侵袭能力降低，细胞周期分析提示G0/G1期的细胞比例增高，S期细胞明显减少，细胞凋亡率明显增加， 且PI 3K、p-Akt及p-mTOR的蛋白水平明显降低（P<0.05）。结论 上调结肠癌HCT-116细胞中miR-7表达能抑制肿瘤细胞的增殖与侵袭，其机制可能与抑制PI3K/AKT信号通路有关
%K miR-7
%K 结肠癌
%K 细胞增殖
%K 凋亡
%K PI3K/AKT信号通路
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%K Advances in Role of Cyclooxygenase 2 in Development and Progression of Cancer
%K Effects of miR-144 on Proliferation
%K Migration
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%K Effect of ANLN Gene Silencing on Invasion
%K Migration
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%K Effects of Pachymaran on Proliferation
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%K Anti-tumor Effects of Chlorogenic Acid on Human Glioblastoma Cell Lines U251 and Related Pro-apoptotic Mechanism
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%U http://www.zlfzyj.com/CN/abstract/abstract9331.shtml