肿瘤相关巨噬细胞促进宫颈癌侵袭转移的机制研究
DOI: 10.3971/j.issn.1000-8578.2019.18.0769
严瑞明,
余艳红,
吴湘光,
吴砂,
周琛斐,
张延梅,
梁罗娇,
梁莉,
王薇,
钟梅,
陈晓静,
魏文斐
Keywords: 宫颈癌,肿瘤微环境（T M E）,肿瘤相关巨噬细胞（TAMs）,侵袭,迁移,Blockade of Radiation-induced EGFR Nuclear Transport Enhances Radiosensitivity of Human Cervical Cancer,miR-601 Suppresses Migration and Invasion of Non-small Cell lung Cancer Cells by Targeting MMP-17,Effects of Sanguinarine on Invasion, Migration and Wnt/β-catenin Signaling Pathway of Lung Adenocarcinoma Cells,Expression and Functional of LncRNA XIST in MCF-7 Breast Cancer Cells,Effect of Down-regulation of HOXB7 Expression on Proliferation, Migration and Invasion Abilities of Hepatoma Cells,Effects of miR-144 on Proliferation, Migration, Invasion and PI3K Pathway of Pancreatic Cancer SW1990 Cells,Effect of ANLN Gene Silencing on Invasion, Migration, Proliferation and Apoptosis of Gastric Cancer Cells,G6PD对大肠癌细胞生长侵袭的影响及其与HKⅡ的相关性,Effects of MicroRNA-4465 on Apoptosis and Invasion of Colon Cancer Cells,Comparison of Survival and Postoperative Complication of Stage Ⅰb2-Ⅱa2 Cervical Cancer Patients Among Three Kinds of Therapeutic Regimens,Effect of FBXL20 on Proliferation, Migration and Invasion of Gastric Cancer Cells,Expression and Correlation of miR-135a-5p, GATA3 and STAT3 in Cervical Cancer,Ampelopsis Regulates Invasive Activity and Invasion Gene Expression of Hepatocellular Carcinoma HepG2 Cell Line Through Dermcidin Protein,Expression of Circular RNA ciRS-7 and Its Effect on Invasion and Migration of Triplenegative reast Cancer Cells,Role of Gadd45 Protein Family in Tumorigenesis and Development
Abstract:
摘要 目的 研究肿瘤相关巨噬细胞（tumor-associated macrophages, TAMs）对宫颈癌侵袭转移的影响及分子机制。方法 免疫组织化学法检测宫颈病变组织TAMs的浸润情况。对人单核巨噬细胞系THP1进行体外诱导分化，使其转化为M2型TAMs。取TAMs上清液刺激宫颈癌细胞SiHa和C33a。划痕法、Transwell法分别检测TAMs对宫颈癌细胞系迁移和侵袭能力的影响。Western blot检测刺激后宫颈癌细胞上皮间质转化进程及MMP-9的表达。结果 TAMs浸润数目与宫颈病变进展呈正相关。TAMs上清液刺激后，SiHa和C33a细胞出现间质样改变，而且迁移和侵袭能力显著增强（均P<0.5）。TAMs可下调E-Cadherin的表达，上调N-Cadherin、Vimentin及MMP-9的表达。结论 TAMs浸润与宫颈上皮恶性转化和进展密切相关，TAMs可能通过上调MMP-9的表达促进宫颈癌细胞侵袭转移
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