%0 Journal Article
%T 肿瘤相关巨噬细胞促进宫颈癌侵袭转移的机制研究
%A 严瑞明
%A 余艳红
%A 吴湘光
%A 吴砂
%A 周琛斐
%A 张延梅
%A 梁罗娇
%A 梁莉
%A 王薇
%A 钟梅
%A 陈晓静
%A 魏文斐
%J 肿瘤防治研究
%D 2019
%R 10.3971/j.issn.1000-8578.2019.18.0769
%X 摘要 目的 研究肿瘤相关巨噬细胞（tumor-associated macrophages, TAMs）对宫颈癌侵袭转移的影响及分子机制。方法 免疫组织化学法检测宫颈病变组织TAMs的浸润情况。对人单核巨噬细胞系THP1进行体外诱导分化，使其转化为M2型TAMs。取TAMs上清液刺激宫颈癌细胞SiHa和C33a。划痕法、Transwell法分别检测TAMs对宫颈癌细胞系迁移和侵袭能力的影响。Western blot检测刺激后宫颈癌细胞上皮间质转化进程及MMP-9的表达。结果 TAMs浸润数目与宫颈病变进展呈正相关。TAMs上清液刺激后，SiHa和C33a细胞出现间质样改变，而且迁移和侵袭能力显著增强（均P<0.5）。TAMs可下调E-Cadherin的表达，上调N-Cadherin、Vimentin及MMP-9的表达。结论 TAMs浸润与宫颈上皮恶性转化和进展密切相关，TAMs可能通过上调MMP-9的表达促进宫颈癌细胞侵袭转移
%K 宫颈癌
%K 肿瘤微环境（T M E）
%K 肿瘤相关巨噬细胞（TAMs）
%K 侵袭
%K 迁移
%K Blockade of Radiation-induced EGFR Nuclear Transport Enhances Radiosensitivity of Human Cervical Cancer
%K miR-601 Suppresses Migration and Invasion of Non-small Cell lung Cancer Cells by Targeting MMP-17
%K Effects of Sanguinarine on Invasion
%K Migration and Wnt/β-catenin Signaling Pathway of Lung Adenocarcinoma Cells
%K Expression and Functional of LncRNA XIST in MCF-7 Breast Cancer Cells
%K Effect of Down-regulation of HOXB7 Expression on Proliferation
%K Migration and Invasion Abilities of Hepatoma Cells
%K Effects of miR-144 on Proliferation
%K Migration
%K Invasion and PI3K Pathway of Pancreatic Cancer SW1990 Cells
%K Effect of ANLN Gene Silencing on Invasion
%K Migration
%K Proliferation and Apoptosis of Gastric Cancer Cells
%K G6PD对大肠癌细胞生长侵袭的影响及其与HKⅡ的相关性
%K Effects of MicroRNA-4465 on Apoptosis and Invasion of Colon Cancer Cells
%K Comparison of Survival and Postoperative Complication of Stage Ⅰb2-Ⅱa2 Cervical Cancer Patients Among Three Kinds of Therapeutic Regimens
%K Effect of FBXL20 on Proliferation
%K Migration and Invasion of Gastric Cancer Cells
%K Expression and Correlation of miR-135a-5p
%K GATA3 and STAT3 in Cervical Cancer
%K Ampelopsis Regulates Invasive Activity and Invasion Gene Expression of Hepatocellular Carcinoma HepG2 Cell Line Through Dermcidin Protein
%K Expression of Circular RNA ciRS-7 and Its Effect on Invasion and Migration of Triplenegative reast Cancer Cells
%K Role of Gadd45 Protein Family in Tumorigenesis and Development
%U http://www.zlfzyj.com/CN/abstract/abstract9350.shtml