FBXL20对胃癌细胞增殖、迁移及侵袭的影响
DOI: 10.3971/j.issn.1000-8578.2019.18.1923
Keywords: 胃癌,FBXL20,Wnt通路,增殖,迁移,侵袭,miR-601 Suppresses Migration and Invasion of Non-small Cell lung Cancer Cells by Targeting MMP-17,Effects of Sanguinarine on Invasion, Migration and Wnt/β-catenin Signaling Pathway of Lung Adenocarcinoma Cells,Expression and Functional of LncRNA XIST in MCF-7 Breast Cancer Cells,Autophagy Related Protein 5 Regulates Colon Cancer Cell Senescence Through p53/p21 Pathway,Mechanism of miRNA-451 Regulating Resistance of Gastric Cancer Cells to 5-Fu Through MRP,Effect of Da Vinci Robot Radical Gastrectomy on Gastrointestinal Motility and Gastrointestinal Hormones of Gastric Cancer Patients,Effect of Down-regulation of HOXB7 Expression on Proliferation, Migration and Invasion Abilities of Hepatoma Cells,Effects of miR-144 on Proliferation, Migration, Invasion and PI3K Pathway of Pancreatic Cancer SW1990 Cells,Effect of ANLN Gene Silencing on Invasion, Migration, Proliferation and Apoptosis of Gastric Cancer Cells,G6PD对大肠癌细胞生长侵袭的影响及其与HKⅡ的相关性,Related Factors and Risk Model Prediction of Pneumonia After ESD in Patients with Early Gastric Cancer,Effects of MicroRNA-4465 on Apoptosis and Invasion of Colon Cancer Cells,Mechanism of D-bifunctional Protein Promoting Formation of Hepatocellular Carcinoma in Rat via STAT3,Regulatory Effect of Astragaloside A on Proliferation and Apoptosis of Human Colon Cancer SW480 Cell Line,Downregulation of Skp2 Expression Inhibits Malignant Phenotype of Osteosarcoma U2OS Cells
Abstract:
摘要 目的 探讨FBXL20在胃癌细胞中的表达及其在胃癌细胞增殖、迁移和侵袭中的作用和机制。方法 Western blot检测人胃癌细胞系（BGC-823、AGS、MKN-45）及胃黏膜上皮细胞系（GES-1）中FBXL20蛋白的表达。靶向FXBL20 shRNA慢病毒载体抑制AGS细胞中FBXL20表达，CCK-8实验检测细胞增殖能力，Transwell实验检测细胞迁移和侵袭能力。Western blot检测抑制FBXL20后Wnt通路中相关蛋白水平变化。结果 与胃黏膜上皮细胞系相比，胃癌细胞系中FBXL20的表达明显增加（P<0.05）。与对照组相比，抑制FBXL20的AGS细胞在增殖、迁移和侵袭能力方面明显降低（P<0.05）。抑制FBXL20能降低GSK-3β的泛素化降解，导致Wnt通路中GSK-3β、p-GSK-3βTyr216的表达增加，而β-catenin、p-β-catenin、Cyclin D1及E-cadherin的表达降低。结论 FBXL20可能通过调控Wnt通路促进胃癌细胞的增殖、迁移和侵袭
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