%0 Journal Article
%T FBXL20对胃癌细胞增殖、迁移及侵袭的影响
%A 刘江
%A 韩娟
%A 黄梅
%J 肿瘤防治研究
%D 2019
%R 10.3971/j.issn.1000-8578.2019.18.1923
%X 摘要 目的 探讨FBXL20在胃癌细胞中的表达及其在胃癌细胞增殖、迁移和侵袭中的作用和机制。方法 Western blot检测人胃癌细胞系（BGC-823、AGS、MKN-45）及胃黏膜上皮细胞系（GES-1）中FBXL20蛋白的表达。靶向FXBL20 shRNA慢病毒载体抑制AGS细胞中FBXL20表达，CCK-8实验检测细胞增殖能力，Transwell实验检测细胞迁移和侵袭能力。Western blot检测抑制FBXL20后Wnt通路中相关蛋白水平变化。结果 与胃黏膜上皮细胞系相比，胃癌细胞系中FBXL20的表达明显增加（P<0.05）。与对照组相比，抑制FBXL20的AGS细胞在增殖、迁移和侵袭能力方面明显降低（P<0.05）。抑制FBXL20能降低GSK-3β的泛素化降解，导致Wnt通路中GSK-3β、p-GSK-3βTyr216的表达增加，而β-catenin、p-β-catenin、Cyclin D1及E-cadherin的表达降低。结论 FBXL20可能通过调控Wnt通路促进胃癌细胞的增殖、迁移和侵袭
%K 胃癌
%K FBXL20
%K Wnt通路
%K 增殖
%K 迁移
%K 侵袭
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%U http://www.zlfzyj.com/CN/abstract/abstract9429.shtml