携带人端粒酶反转录酶启动子的溶瘤腺病毒RCA-TERT-Ad35对乳腺癌干细胞的靶向作用
DOI: 10.3971/j.issn.1000-8578.2019.18.1987
Keywords: 乳腺癌干细胞,人端粒酶反转录酶,溶瘤腺病毒,基因治疗,New NGR-targeted shRNA-loaded Liposome Suppresses Glioma Growth,Tanshinone ⅡA Enhances Chemosensitivity of Breast Cancer Cells to Doxorubicin and Related Mechanism,Effects of Antisense Oligonucleotides Against miRNA-21 on Growth of Human Colon Cancer Cells in vivo,Regulations and Roles of Breast Cancer Stem Cells,Effect of 5-Azacytidine on hTERT mRNA Expression and Telomerase Activity in Human Nasopharyngeal Carcinoma Cells,stathmin Silencing Increases Sensitivity of Esophageal Squamous Cell Carcinoma Cells to Paclitaxel,Inhibitory Activity of T-VISA-BikDD Plasmid on Tumor Cells in Vitro,Progress in TRAIL-based Therapy for Glioma,Inhibitory Effect of Celecoxib on Triple-negative Breast Cancer Stem Cells in vitro,Construction of IL-24-TRAIL Vector and Its Infl uences on Migration and Apoptosis of#br# Breast Cancer Stem Cells,Effect of hTERTC27 Overexpression on Prolifteration and Apoptosis of Nasopharyngeal Carcinoma C666-1 Cell Line,Effect of Oncolytic Adenoviruses Ad-MK Combined with Irradiation on Bladder Cancer,Study of Targeting Ability of Folic Acid/polyamide-amine As miR-7 Vector to Glioma,Isolation and Primary Culture and Biological Characteristics Analysis of ALDH+ Breast Cancer Stem Cells
Abstract:
摘要 目的 探讨携带人端粒酶反转录酶启动子的溶瘤腺病毒RCA-TERT-Ad35在靶向乳腺癌干细胞中的作用。方法 乳腺癌细胞系MCF-7以球体形式在加入生长因子的无血清培养基中生长。同时构建携带TERT启动子的溶瘤腺病毒RCA-TERT-Ad35，并通过溶瘤、MTT及体内抗肿瘤实验观察RCA-TERT-Ad35对干细胞样癌细胞的杀伤效果。结果 与亲代细胞相比，MCF-7球体细胞显示出干细胞特性，且MCF-7球体细胞中hTERT基因过表达，其对细胞毒性剂紫杉醇具有耐药性。溶瘤腺病毒RCA-TERT-Ad35在TERT过表达的细胞中进行复制并抑制细胞生长。在裸鼠移植瘤模型中，与RCA-Ad35相比，RCA-TERT-Ad35显著抑制肿瘤生长并改善小鼠的存活状态。结论 溶瘤腺病毒RCA-TERT-Ad35可优先杀伤TERT过表达的乳腺癌干细胞
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