%0 Journal Article
%T 携带人端粒酶反转录酶启动子的溶瘤腺病毒RCA-TERT-Ad35对乳腺癌干细胞的靶向作用
%A 于可心
%A 张松男
%A 权香兰
%A 李颖
%A 王炜川
%A 金文彪
%A 金永民
%A 陈璐璐
%J 肿瘤防治研究
%D 2019
%R 10.3971/j.issn.1000-8578.2019.18.1987
%X 摘要 目的 探讨携带人端粒酶反转录酶启动子的溶瘤腺病毒RCA-TERT-Ad35在靶向乳腺癌干细胞中的作用。方法 乳腺癌细胞系MCF-7以球体形式在加入生长因子的无血清培养基中生长。同时构建携带TERT启动子的溶瘤腺病毒RCA-TERT-Ad35，并通过溶瘤、MTT及体内抗肿瘤实验观察RCA-TERT-Ad35对干细胞样癌细胞的杀伤效果。结果 与亲代细胞相比，MCF-7球体细胞显示出干细胞特性，且MCF-7球体细胞中hTERT基因过表达，其对细胞毒性剂紫杉醇具有耐药性。溶瘤腺病毒RCA-TERT-Ad35在TERT过表达的细胞中进行复制并抑制细胞生长。在裸鼠移植瘤模型中，与RCA-Ad35相比，RCA-TERT-Ad35显著抑制肿瘤生长并改善小鼠的存活状态。结论 溶瘤腺病毒RCA-TERT-Ad35可优先杀伤TERT过表达的乳腺癌干细胞
%K 乳腺癌干细胞
%K 人端粒酶反转录酶
%K 溶瘤腺病毒
%K 基因治疗
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%U http://www.zlfzyj.com/CN/abstract/abstract9504.shtml