抑制FoxM1基因表达对甲状腺乳头状癌TPC-1细胞糖酵解途径的影响
DOI: 10.3971/j.issn.1000-8578.2019.18.0081
Keywords: 甲状腺乳头状癌,TPC-1细胞,FoxM1基因,糖酵解,Apatinib Suppresses Proliferation and Induced Apoptosis of Human Breast Cancer Cell Line MDA-MB-231 Through Glycolytic Inhibition,Progress of Inhibiting Embden-Meyerhof-Parnas in Treatment of Colorectal Cancer,CCNG2 Expression and Its Effect on Proliferation and Apoptosis of Thyroid Papillary Cancer K1 Cells,Research Progress of Tumor Metabolism for Antitumor Drugs,Pyruvate Dehydrogenase and Tumor,Effects of Human Chorionic Gonadotropin on Growth of Papillary Thyroid Carcinoma,Correlation of Moesin and E-cadherin with Invasion of Papillary Thyroid Carcinoma,Expressions and Clinical Significance of AnnexinⅡand S100A4 in Papillary Thyroid Carcinoma,Relationship between Expressions of VEGF,MMP-9,COX-2 and Lymph Node Metastasis and Angiogenesis in Papillary Thyroid Carcinoma,Expression andSignificanceofREGγ andp21 Proteins in Papillary Thyroid Carcinoma Tissues,Diagnostic Significance of Galectin-3 in Early Phase of Thyroid Papillary Carcinoma,Expression and Clinical Significance of P-GP170, LRP and Ki-67 in Thyroid Carcinoma
Abstract:
摘要 目的 探讨抑制FoxM1基因表达对甲状腺乳头状癌（PTC）细胞葡萄糖摄取和乳酸产量的影响及FoxM1与PTC细胞糖酵解途径的相关性。方法 用慢病毒rLv-hFoxM1转染PTC TPC-1细胞，检测转染后的TPC-1细胞中FoxM1 mRNA及蛋白表达水平的变化；应用噻唑蓝（MTT）比色法检测抑制FoxM1基因对TPC-1细胞活性的影响；检测转染慢病毒rLv-hFoxM1后的TPC-1细胞在葡萄糖摄取量和乳酸产量方面的变化以及糖酵解途径关键酶己糖激酶1（HK1）、己糖激酶2（HK2），葡萄糖转运体蛋白1（Glut1）含量的变化。结果 在TPC-1细胞中，慢病毒rLv-hFoxM1转染可明显抑制TPC-1细胞中FoxM1基因mRNA及蛋白水平的表达，且转染慢病毒rLv-hFoxM1后TPC-1细胞的活性明显下降（P<0.05），随着FoxM1基因表达降低，TPC-1细胞的葡萄糖摄取量及乳酸产量均明显下降（P<0.01），抑制FoxM1基因后，其葡萄糖转运蛋白（Glut1）、己糖激酶1（HK1）和己糖激酶（HK2）含量明显降低。结论 抑制FoxM1基因的表达能降低PTC细胞的糖酵解水平
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