%0 Journal Article
%T 抑制FoxM1基因表达对甲状腺乳头状癌TPC-1细胞糖酵解途径的影响
%A 徐卫燕
%A 李永平
%A 蒋晓飞
%A 袁浩
%A 闵志均
%A 陈进宏
%J 肿瘤防治研究
%D 2019
%R 10.3971/j.issn.1000-8578.2019.18.0081
%X 摘要 目的 探讨抑制FoxM1基因表达对甲状腺乳头状癌（PTC）细胞葡萄糖摄取和乳酸产量的影响及FoxM1与PTC细胞糖酵解途径的相关性。方法 用慢病毒rLv-hFoxM1转染PTC TPC-1细胞，检测转染后的TPC-1细胞中FoxM1 mRNA及蛋白表达水平的变化；应用噻唑蓝（MTT）比色法检测抑制FoxM1基因对TPC-1细胞活性的影响；检测转染慢病毒rLv-hFoxM1后的TPC-1细胞在葡萄糖摄取量和乳酸产量方面的变化以及糖酵解途径关键酶己糖激酶1（HK1）、己糖激酶2（HK2），葡萄糖转运体蛋白1（Glut1）含量的变化。结果 在TPC-1细胞中，慢病毒rLv-hFoxM1转染可明显抑制TPC-1细胞中FoxM1基因mRNA及蛋白水平的表达，且转染慢病毒rLv-hFoxM1后TPC-1细胞的活性明显下降（P<0.05），随着FoxM1基因表达降低，TPC-1细胞的葡萄糖摄取量及乳酸产量均明显下降（P<0.01），抑制FoxM1基因后，其葡萄糖转运蛋白（Glut1）、己糖激酶1（HK1）和己糖激酶（HK2）含量明显降低。结论 抑制FoxM1基因的表达能降低PTC细胞的糖酵解水平
%K 甲状腺乳头状癌
%K TPC-1细胞
%K FoxM1基因
%K 糖酵解
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%U http://www.zlfzyj.com/CN/abstract/abstract9367.shtml